Electrically Controlled Molecular Recognition Harnessed to Activate a Cellular Response

被引：9

作者：

Artzy-Schnirman, Arbel ^{[1
,2
,3
]}

Blat, Dan ^{[4
]}

Talmon, Yael ^{[1
,2
,3
]}

Fishler, Rami ^{[1
,3
]}

Gertman, Dori ^{[2
]}

Oren, Ravit ^{[2
]}

Wolchinsky, Ron ^{[2
]}

Waks, Tova ^{[4
]}

Benhar, Itai ^{[5
]}

Eshhar, Zelig ^{[4
]}

Sivan, Uri ^{[1
,3
]}

Reiter, Yoram ^{[2
,3
]}

机构：

[1] Technion Israel Inst Technol, Dept Phys, IL-32000 Haifa, Israel

[2] Technion Israel Inst Technol, Dept Biol, IL-32000 Haifa, Israel

[3] Technion Israel Inst Technol, Russell Berrie Nanotechnol Inst, IL-32000 Haifa, Israel

[4] Weizmann Inst Sci, Dept Immunol, IL-76100 Rehovot, Israel

[5] Tel Aviv Univ, George S Wise Fac Life Sci, Dept Mol Microbiol & Biotechnol, IL-69978 Tel Aviv, Israel

来源：

NANO LETTERS | 2011年 / 11卷 / 11期

基金：

以色列科学基金会;

关键词：

T-cell chimeric receptor; electrical activation; antibody engineering; bioelectronic interface; electronic antigen; ANTIGEN;

D O I：

10.1021/nl202971r

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Seamless embedment of electronic devices in biological systems is expected to add the outstanding computing power; memory, and speed of electronics to the biochemical toolbox of nature. Such amalgamation requires transduction of electronic signals into biochemical cues that affect cells. Inspired by biology, where pathways are directed by molecular recognition, we propose and demonstrate a generic electrical-to-biological transducer comprising a two-state electronic antigen and a chimeric cell receptor engineered to bind the antigen exclusively in its "on" state. T-cells expressing these receptors remain inactivated with the antigen in its "off" state. Switching the antigen to its "on" state by an electrical signal leads to its recognition by the T-cells and correspondingly to cell activation.

引用

页码：4997 / 5001

页数：5

共 14 条

[1] A Two-State Electronic Antigen and An Antibody Selected to Discriminate Between These States [J].