Polymorphic microsatellites developed by cross-species amplifications in common pheasant breeds

被引：26

作者：

Baratti, M ^{[1
]}

Alberti, A

Groenen, M

Veenendaal, T

Fulgheri, FD

机构：

[1] Univ Florence, Dept Anim Biol & Genet, I-50121 Florence, Italy

[2] Natl Wildlife Inst, Bologna, Italy

[3] Univ Wageningen & Res Ctr, Dept Anim Sci, Wageningen, Netherlands

来源：

ANIMAL GENETICS | 2001年 / 32卷 / 04期

关键词：

avian; common pheasant; cross-species amplification; microsatellites; Phasianiidae;

D O I：

10.1046/j.1365-2052.2001.00767.x

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Genetic variability was analysed in two common breeds of pheasant (Phasianus colchicus L. 1758) by means of cross-species amplifications of microsatellite loci: 154 chicken, Gallus gallus and 32 turkey, Meleagris gallopavo. primers were tested for amplification of pheasant DNA. Thirty-six primers (25 specific for chicken and 11 for turkey) amplified pheasant DNA. Fifteen markers yielded specific products and were tested for polymorphism. Eight of them (55%) were polymorphic, with an average polymorphism of two alleles per locus. Specific polymerase chain reaction (PCR) products were sequenced; repeats were found in 11 of the 15 markers, although only two loci showed the same repeat and could be homologous to chicken ones.

引用

页码：222 / 225

页数：4

共 15 条

[1] PHYLOGENETIC ANALYSIS - MODELS AND ESTIMATION PROCEDURES [J].