Mass spectrometry of single-stranded restriction fragments captured by an undigested complementary sequence

被引:9
作者
Chiu, Norman H. L. [1 ,2 ]
Tang, Kai [1 ]
Yip, Ping [1 ]
Braun, Andreas [1 ]
Koster, Hubert [1 ]
Cantor, Charles R. [1 ,2 ]
机构
[1] Sequenom Inc, San Diego, CA 92121 USA
[2] Boston Univ, Ctr Adv Biotechnol, Boston, MA 02215 USA
关键词
D O I
10.1093/nar/28.8.e31
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this report, we describe a simple and accurate method to analyze restriction fragments using matrixassisted laser desorption/ionization time-of-flight mass spectrometry. The two complementary strands of restriction fragments are separated through hybridization to a capture probe, which is a single-stranded undigested fragment. Using the biotinstreptavidin linkage, the hybrid is immobilized on streptavidin-coated magnetic beads. After conditioning the captured restriction fragments, they are eluted from the probe and their molecular weights are determined. The proposed method greatly improves the quality, and reduces the complexity of the mass spectrum by analyzing only one of the complementary strands of restriction fragments.
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页数:4
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