Interaction of cAMP receptor protein from Escherichia coli with cAMP and DNA studied by differential scanning calorimetry

被引：6

作者：

Blaszczyk, U ^{[1
]}

Wasylewski, Z ^{[1
]}

机构：

[1] Jagiellonian Univ, Dept Phys Biochem, Fac Biotechnol, PL-30387 Krakow, Poland

来源：

JOURNAL OF PROTEIN CHEMISTRY | 2003年 / 22卷 / 03期

关键词：

cyclic AMP receptor protein; differential scanning calorimetry; transcription activation; Escherichia coli;

D O I：

10.1023/A:1025024604677

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cyclic AMP receptor protein (CRP) regulates the expression of many genes in Escherichia coli. The protein is a homodimer, and each monomer is folded into two distinct structural domains. In this study, we have used differential scanning calorimetry (DSC) and circular dichroism (CD) to measure the enthalpy change and melting temperature of the apo-CRP and CRP complexes with cAMP or DNA sequences lac, gal, and palindromic ICAP. DSC and CD measurements showed irreversible thermal denaturation process of CRP. Enthalpy of dissociation of the protein-DNA complex, as measured by DSC, depends on the DNA sequence. The thermal transition of the protein in CRP-DNA complexes, measured by CD, indicates that the protein stability in the complex is also DNA sequence-dependent.

引用

页码：285 / 293

页数：9

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