Novel homeodomain-interacting protein kinase family member, HIPK4, phosphorylates human p53 at serine 9

被引：36

作者：

Arai, Shigeki ^{[1
]}

Matsushita, Akio ^{[1
]}

Du, Kun ^{[1
]}

Yagi, Ken ^{[2
]}

Okazaki, Yasushi ^{[2
]}

Kurokawa, Riki ^{[1
]}

机构：

[1] Saitama Med Univ, Res Ctr Genom Med, Div Gene Struct & Funct, Kawagoe, Saitama, Japan

[2] Saitama Med Univ, Res Ctr Genom Med, Div Funct Genom & Syst Med, Kawagoe, Saitama, Japan

来源：

FEBS LETTERS | 2007年 / 581卷 / 29期

关键词：

kinase; transcription; tumor suppressor; p53; apoptosis;

D O I：

10.1016/j.febslet.2007.11.022

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe here the cloning and characterization of a novel mouse homeodomain-interacting protein kinase (HIPK)-like gene, Hipk4. Hipk4 is expressed in lung and in white adipose tissue and encodes a 616 amino acid protein that includes a serine/threonine kinase domain. We demonstrate that HIPK4 could phosphorylate human p53 protein at serine 9, both in vitro and in vivo. Among known p53-responsive promoters, activity of the human survivin promoter, which is repressed by p53, was decreased by HIPK4 in p53 functional A549 cells. Human BCL2-associated X protein-promoter activity was not affected. These findings suggest that phosphorylation of p53 at serine 9 is important for p53 mediated transcriptional repression. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：5649 / 5657

页数：9

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