Novel putative saposin-like proteins of Entamoeba histolytica different from amoebapores

被引：21

作者：

Bruhn, H ^{[1
]}

Leippe, M ^{[1
]}

机构：

[1] Bernhard Nocht Inst Trop Med, D-20359 Hamburg, Germany

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2001年 / 1514卷 / 01期

基金：

美国国家卫生研究院;

关键词：

amoebapore; saposin-like protein; membrane interaction; pore-forming; antimicrobial peptide; Entamoeba histolytica;

D O I：

10.1016/S0005-2736(01)00345-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Amoebapores, the pore-forming proteins of Entamoeba histolytica, have been shown to play a pivotal role in the pathogenicity of the protozoan parasite. They belong to the functionally diverse family of saposin-like proteins (SAPLIPs) characterized by a conserved pattern of cysteine residues and the ability to interact with lipids. Here, we report the identification of genomic sequences encoding presumably novel SAPLIPs in E. histolytica and classify them in the structural and functional context provided by known family members. The genes of altogether 15 SAPLIPs are transcribed in the axenically cultured trophozoites as evidenced by reverse transcriptase-polymerase chain reaction. Interestingly, a remarkable sequence variety with a strong resemblance to that of known, functionally diverse SAPLIPs is present in this archaic, unicellular organism. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：14 / 20

页数：7

共 35 条

[1] NK-LYSIN, A NOVEL EFFECTOR PEPTIDE OF CYTOTOXIC T-CELLS AND NK-CELLS - STRUCTURE AND CDNA CLONING OF THE PORCINE FORM, INDUCTION BY INTERLEUKIN-2, ANTIBACTERIAL AND ANTITUMOR-ACTIVITY [J].