Adiponectin is synthesized and secreted by human and murine cardiomyocytes

被引：302

作者：

Piñeiro, R

Iglesias, MJ

Gallego, R

Raghay, K

Eiras, S

Rubio, J

Diéguez, C

Gualillo, O

González-Juanatey, JR

Lago, F

机构：

[1] Univ Santiago de Compostela, Lab Invest 1, Area Invest & Docencia, Hosp Clin,Unidad Invest Serv Cardiol, Santiago De Compostela 15706, Spain

[2] Univ Santiago de Compostela, Dept Ciencias Morfol, Santiago De Compostela 15706, Spain

[3] Univ Santiago de Compostela, Dept Fisiol, Santiago De Compostela 15706, Spain

[4] Univ Santiago de Compostela, Unidad Invest Serv Reumatol, Hosp Clin, Santiago De Compostela 15706, Spain

来源：

FEBS LETTERS | 2005年 / 579卷 / 23期

关键词：

cardiomyocyte; adiponectin; energy metabolism; AMP-activated protein kinase; metabolic syndrome;

D O I：

10.1016/j.febslet.2005.07.098

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Adiponectin is thought to play a decisive role in the relationships among obesity, insulin resistance and cardiovascular risk. This study investigated whether cardiomyocytes synthesize and secrete adiponectin, and the effects of this hormone on cardiac cells. RT-PCR showed that mouse, rat and human cardiomyocytes produced mRNA for adiponectin and adiponectin receptors 1 and 2. Immunohistochemistry confirmed the presence of adiponectin in the cytoplasm of cultured cardiomyocytes, and radioimmunoassay showed that these cells secreted adiponectin into the culture medium. Exogenous adiponectin enhanced glucose and fatty acid uptake and induced AMPK phosphorylation in cultured cardiomyocytes. Our results demonstrate that adiponectin is synthesized and secreted by isolated murine and human cardiomyocytes, and suggest that the local production of this hormone by cardiomyocytes could be involved in the regulation of cardiac metabolism and function. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：5163 / 5169

页数：7

共 25 条

[1] Fat depot-specific expression of adiponectin is impaired in Zucker fatty rats [J].

Altomonte, J ;

Harbaran, S ;

Richter, A ;

Dong, HJ .

METABOLISM-CLINICAL AND EXPERIMENTAL, 2003, 52 (08) :958-963

[2] Adiponectin and its receptors are expressed in bone-forming cells [J].

Berner, HS ;

Lyngstadaas, SP ;

Spahr, A ;

Monjo, M ;

Thommesen, L ;

Drevon, CA ;

Syversen, U ;

Reseland, JE .

BONE, 2004, 35 (04) :842-849

[3] Induction of adiponectin in skeletal muscle by inflammatory cytokines:: In vivo and in vitro studies [J].

Delaigle, AM ;

Jonas, JC ;

Bauche, IB ;

Cornu, O ;

Brichard, SM .

ENDOCRINOLOGY, 2004, 145 (12) :5589-5597

[4] Adiponectin: A novel adipokine linking adipocytes and vascular function [J].

Goldstein, BJ ;

Scalia, R .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2004, 89 (06) :2563-2568

[5] Doxazosin induces apoptosis in cardiomyocytes cultured in vitro by a mechanism that is independent of α1-adrenergic blockade [J].

González-Juanatey, JR ;

Iglesias, MJ ;

Alcaide, C ;

Piñeiro, R ;

Lago, F .

CIRCULATION, 2003, 107 (01) :127-131

[6] The role of the adipocyte hormone adiponectin in cardiovascular disease [J].

Hug, C ;

Lodish, HF .

CURRENT OPINION IN PHARMACOLOGY, 2005, 5 (02) :129-134

[7] Changes in the distribution pattern of gelatin-binding protein of 28 kDa (adiponectin) in myocardial remodelling after ischaemic injury [J].

Ishikawa, Y ;

Akasaka, Y ;

Ishii, T ;

Yoda-Murakami, M ;

Choi-Miura, NH ;

Tomita, M ;

Ito, K ;

Zhang, L ;

Akishima, Y ;

Ishihara, M ;

Muramatsu, M ;

Taniyama, M .

HISTOPATHOLOGY, 2003, 42 (01) :43-52

[8] Hypoadiponectinemia is an independent risk factor for hypertension [J].

Iwashima, Y ;

Katsuya, T ;

Ishikawa, K ;

Ouchi, N ;

Ohishi, M ;

Sugimoto, K ;

Fu, YX ;

Motone, M ;

Yamamoto, K ;

Matsuo, A ;

Ohashi, K ;

Kihara, S ;

Funahashi, T ;

Rakugi, H ;

Matsuzawa, Y ;

Ogihara, T .

HYPERTENSION, 2004, 43 (06) :1318-1323

[9] Role of the nitric oxide pathway in AMPK-mediated glucose uptake and GLUT4 translocation in heart muscle [J].

Li, J ;

Hu, XY ;

Selvakumar, P ;

Russell, RR ;

Cushman, SW ;

Holman, GD ;

Young, LH .

AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 2004, 287 (05) :E834-E841

[10] Adiponectin gene expression in subcutaneous adipose tissue of obese women in response to short-term very low calorie diet and refeeding [J].

Liu, YM ;

Lacorte, JM ;

Viguerie, N ;

Poitou, C ;

Pelloux, V ;

Guy-Grand, B ;

Coussieu, C ;

Langin, D ;

Basdevant, A ;

Clément, K .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2003, 88 (12) :5881-5886

← 1 2 3 →