Microfluidic electrocapture for separation of peptides

被引：34

作者：

Astorga-Wells, J ^{[1
]}

Vollmer, S ^{[1
]}

Tryggvason, S ^{[1
]}

Bergman, T ^{[1
]}

Jörnvall, H ^{[1
]}

机构：

[1] Karolinska Inst, Dept Med Biochem & Biophys, SE-17177 Stockholm, Sweden

来源：

ANALYTICAL CHEMISTRY | 2005年 / 77卷 / 22期

关键词：

D O I：

10.1021/ac050931z

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A separation method based on electroimmobilization and sequential release of captured molecules is reported. A microfluidic electrocapture device is utilized to immobilize peptides in a microflow stream. After capture, the electric field is decreased in a stepwise manner, causing sequential release of the captured peptides according to their electrophoretic mobility. Tryptic peptides were separated and analyzed by matrix-assisted laser desorption/ionization mass spectrometry. The separation power was high enough to increase the ionization yield of several peptides not seen in the unprocessed sample. In addition to separation, simultaneous sample cleanup was demonstrated for peptides obtained by shotgun tryptic digestion of membrane protein extracts.

引用

页码：7131 / 7136

页数：6

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