An improved sample packing device for rapid freeze-trap electron paramagnetic resonance spectroscopy kinetic measurements

被引：31

作者：

Tsai, AL

Berka, V

Kulmacz, RJ

Wu, G

Palmer, G

机构：

[1] Univ Texas, Sch Med, Dept Internal Med, Div Hematol, Houston, TX 77225 USA

[2] Rice Univ, Dept Biochem & Cell Biol, Houston, TX 77251 USA

来源：

ANALYTICAL BIOCHEMISTRY | 1998年 / 264卷 / 02期

关键词：

1 This work was supported by United Public Health Service Grants GM44911; GM21337; GM52170; and Welch C336. 2To whom correspondence should be addressed at Division of Hematology; Department of Internal Medicine; University of Texas Medical School at Houston; P.O; Box; 20708; Houston; TX 77225. 3 Abbreviations used: EPR; electron paramagnetic resonance spectroscopy; Mb; myoglobin;

D O I：

10.1006/abio.1998.2774

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method has been developed for sample packing in rapid freeze-quench electron paramagnetic resonance spectroscopy (EPR) kinetic experiments. Sample particles freeze-quenched in chilled isopentane are filtered under pressure through a stainless steel funnel attached to an EPR tube fitted with a porous disk at its bottom. Isopentane exits through the porous disk and the sample particles can be transferred essentially quantitatively into the receiving EPR tube. This device provides a more predictable, reproducible, and time-saving method for sample packing, enables use of a wider range of how velocity, and allows efficient use of valuable reactants. (C) 1998 Academic Press.

引用

页码：165 / 171

页数：7

共 9 条

[1]

Ballou D. P., 1971, THESIS U MICHIGAN

[2] PRACTICAL RAPID QUENCHING INSTRUMENT FOR STUDY OF REACTION-MECHANISMS BY ELECTRON-PARAMAGNETIC RESONANCE SPECTROSCOPY [J].