Molecular imprinting: Synthesis of polymer particles with antibody-like binding characteristics for glucose oxidase

A phosphate buffer containing a mixture of glucose oxidase, acrylic acid derivatives, N,N'-1,2 dihpdroxpethylene-bis(acrylamide), N,N'-(methylene)-bisacrylamide and surface-modified silica was radically polymerized with (NH4)(2)S2O8. The polymer formed a thin layer around the silica beads. After sieving of these polymer particles, the surface bound protein was eluted. In rebinding assays and enzyme activity tests a specific binding capacity for glucose oxidase of up to 0.557 mu g GOD/100 mg dry weight of polymer particles could be determined. These polymer particles have the potential to be used as specific separation Or dectection material. (C) 1996 Academic Press, Inc.