DNA methylation inhibits expression and transposition of the Neurospora Tad retrotransposon

被引：26

作者：

Zhou, Y ^{[1
]}

Cambareri, EB ^{[1
]}

Kinsey, JA ^{[1
]}

机构：

[1] Univ Kansas, Med Ctr, Dept Microbiol Mol Genet & Immunol, Kansas City, KS 66160 USA

来源：

MOLECULAR GENETICS AND GENOMICS | 2001年 / 265卷 / 04期

基金：

美国国家科学基金会;

关键词：

Neurospora crassa; Tad; cytosine methylation; inhibition of transposition;

D O I：

10.1007/s004380100472

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tad is a LINE-like retrotransposon of the filamentous fungus Neurospora crassa. We have analyzed both expression and transposition of this element using strains with a single copy of Tad located in the 5' noncoding sequences of the am (glutamate dehydrogenase) gene. Tad in this position has been shown to carry a de novo cytosine methylation signal which causes reversible methylation of both Tad and am upstream sequences. Here we find that methylation of the Tad sequences inhibits both Tad expression and transposition. This inhibition can be relieved by the use of 5-azacytidine, a drug which reduces cytosine methylation, or by placing the Tad/am sequences in a dim-2 genetic background.

引用

页码：748 / 754

页数：7

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