Photo-cured hyaluronic acid-based hydrogels containing simvastatin as a bone tissue regeneration scaffold

被引：149

作者：

Bae, Min Soo ^{[1
,2
]}

Yang, Dae Hyeok ^{[1
,2
]}

Lee, Jung Bok ^{[1
,2
]}

Heo, Dong Nyoung ^{[1
,2
]}

Kwon, Yong-Dae ^{[3
]}

Youn, In Chan ^{[4
]}

Choi, Kuiwon ^{[4
]}

Hong, Jong Hyun ^{[5
]}

Kim, Gye Tae ^{[5
]}

Choi, Yong Suk ^{[5
]}

Hwang, Eui Hwan ^{[5
]}

Kwon, Il Keun ^{[1
,2
]}

机构：

[1] Kyung Hee Univ, Sch Dent, Dept Maxillofacial Biomed Engn, Seoul 130701, South Korea

[2] Kyung Hee Univ, Sch Dent, Inst Oral Biol, Seoul 130701, South Korea

[3] Kyung Hee Univ, Sch Dent, Dept Oral & Maxillofacial Surg, Seoul 130701, South Korea

[4] Korea Inst Sci & Technol, Biomed Res Ctr, Seoul 136791, South Korea

[5] Kyung Hee Univ, Sch Dent, Dept Oral & Maxillofacial Radiol, Seoul 130701, South Korea

来源：

BIOMATERIALS | 2011年 / 32卷 / 32期

基金：

新加坡国家研究基金会;

关键词：

Photopolymerization; Hyaluronic acid; Hydrogel; Simvastatin; Bone tissue regeneration; IN-VITRO; PHOTOPOLYMERIZATION; NANOPARTICLES; CELLS;

D O I：

10.1016/j.biomaterials.2011.07.045

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

We describe in this study the positive influences on in vitro and in vivo osteogenesis of photo-cured hyaluronic acid (HA) hydrogels loaded with simvastatin (SIM). Prior to loading SIM, we first characterized the HA hydrogels for their mechanical properties and swelling ratios. The results from this testing indicated that these two factors improved as the substitution degree of 2-aminoethyl methacrylate (AEMA) increased. MTT and live/dead assays showed that the HA hydrogels have good biocompatibility for use as scaffolds for bone tissue regeneration. Moreover, another KIT assay showed that the photo-cured HA hydrogels III fabricated with 30% AEMA (300 mg) conjugated HA (HA-AEMA iii) loaded with between 0.1 and 1 mg of SIM had a similar cytotoxicity as compared to the HA hydrogel III itself. The sustained release of SIM was observed to occur in the HA hydrogel III loaded with 1 mg of SIM. In vitro and in vivo experiments showed that the HA hydrogel III loaded with 1 mg of SIM had a significant influence on osteogenesis. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：8161 / 8171

页数：11

共 28 条

[1]

A novel collagen/hydroxyapatite/poly(lactide-co-ε-caprolactone) biodegradable and bioactive 3D porous scaffold for bone regeneration [J].

Akkouch, Adil ;

Zhang, Ze ;

Rouabhia, Mahmoud .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2011, 96A (04) :693-704

[2]

Influence of the degree of methacrylation on hyaluronic acid hydrogels properties [J].

Bencherif, Sidi A. ;

Srinivasan, Abiraman ;

Horkay, Ferenc ;

Hollinger, Jeffrey O. ;

Matyjaszewski, Krzysztof ;

Washburn, Newell R. .

BIOMATERIALS, 2008, 29 (12) :1739-1749

[3]

Cytocompatibility of UV and visible light photoinitiating systems on cultured NIH/3T3 fibroblasts in vitro [J].

Bryant, SJ ;

Nuttelman, CR ;

Anseth, KS .

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 2000, 11 (05) :439-457

[4]

The effects of scaffold thickness on tissue engineered cartilage in photocrosslinked poly(ethylene oxide) hydrogels [J].

Bryant, SJ ;

Anseth, KS .

BIOMATERIALS, 2001, 22 (06) :619-626

[5]

Photopolymerization in polymer templating [J].

Clapper, Jason D. ;

Sievens-Figueroa, Lucas ;

Guymon, C. Allan .

CHEMISTRY OF MATERIALS, 2008, 20 (03) :768-781

[6]

Injectable pH- and Temperature-Responsive Poly(N-isopropylacrylamide-co-propylacrylic acid) Copolymers for Delivery of Angiogenic Growth Factors [J].

Garbern, Jessica C. ;

Hoffman, Allan S. ;

Stayton, Patrick S. .

BIOMACROMOLECULES, 2010, 11 (07) :1833-1839

[7]

Hyaluronic acid hydrogen for controlled self-renewal and differentiation of human embryonic stem cells [J].

Gerecht, Sharon ;

Burdick, Jason A. ;

Ferreira, Lino S. ;

Townsend, Seth A. ;

Langer, Robert ;

Vunjak-Novakovic, Gordana .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (27) :11298-11303

[8]

Poorly water-soluble drug nanoparticles via an emulsion-freeze-drying approach [J].

Grant, Neil ;

Zhang, Haifei .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2011, 356 (02) :573-578

[9]

Gurkan UA, 2011, TISSUE ENG PT A, V17, P417, DOI [10.1089/ten.tea.2010.0193, 10.1089/ten.TEA.2010.0193]

[10]

Rapidly in situ forming biodegradable robust hydrogels by combining stereocomplexation and photopolymerization [J].

Hiemstra, Christine ;

Zhou, Wei ;

Zhong, Zhiyuan ;

Wouters, Marielle ;

Feijen, Jan .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (32) :9918-9926

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