Portable, Battery-Operated, Low-Cost, Bright Field and Fluorescence Microscope

被引：83

作者：

Miller, Andrew R. ^{[1
]}

Davis, Gregory L.

Oden, Z. Maria ^{[1
]}

Razavi, Mohamad Reza ^{[2
]}

Fateh, Abolfazl ^{[2
]}

Ghazanfari, Morteza ^{[2
]}

Abdolrahimi, Farid ^{[2
]}

Poorazar, Shahin ^{[2
]}

Sakhaie, Fatemeh ^{[2
]}

Olsen, Randall J. ^{[3
]}

Bahrmand, Ahmad Reza ^{[2
,3
]}

Pierce, Mark C. ^{[1
]}

Graviss, Edward A. ^{[3
]}

Richards-Kortum, Rebecca ^{[1
]}

机构：

[1] Rice Univ, Dept Bioengn, Houston, TX 77251 USA

[2] Inst Pasteur, Dept Mycobacteriol, Tehran, Iran

[3] Methodist Hosp, Res Inst, Ctr Mol & Translat Human Infect Dis Res, Houston, TX 77030 USA

来源：

PLOS ONE | 2010年 / 5卷 / 08期

关键词：

TUBERCULOSIS; DIAGNOSIS;

D O I：

10.1371/journal.pone.0011890

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

This study describes the design and evaluation of a portable bright-field and fluorescence microscope that can be manufactured for $240 USD. The microscope uses a battery-operated LED-based flashlight as the light source and achieves a resolution of 0.8 mu m at 1000x magnification in fluorescence mode. We tested the diagnostic capability of this new instrument to identify infections caused by the human pathogen, Mycobacterium tuberculosis. Sixty-four direct, decontaminated, and serially diluted smears were prepared from sputa obtained from 19 patients suspected to have M. tuberculosis infection. Slides were stained with auramine orange and evaluated as being positive or negative for M. tuberculosis with both the new portable fluorescence microscope and a laboratory grade fluorescence microscope. Concordant results were obtained in 98.4% of cases. This highly portable, low cost, fluorescence microscope may be a useful diagnostic tool to expand the availability of M. tuberculosis testing at the point-of-care in low resource settings.

引用

页数：3

共 9 条

[1] Rural poverty and delayed presentation to tuberculosis services in Ethiopia [J].