A multicopy suppressor of a cell cycle defect in S-pombe encodes a heat shock-inducible 40 kDa cyclophilin-like protein

被引:46
作者
Weisman, R
Creanor, J
Fantes, P
机构
[1] UNIV EDINBURGH, INST CELL & MOLEC BIOL, EDINBURGH EH9 3JR, MIDLOTHIAN, SCOTLAND
[2] UNIV EDINBURGH, INST CELL ANIM & POPULAT BIOL, EDINBURGH EH9 3JR, MIDLOTHIAN, SCOTLAND
关键词
cell cycle; cyclophilin; fission yeast; heat shock; S-pombe; CIS-TRANS ISOMERASE; YEAST SCHIZOSACCHAROMYCES-POMBE; STEROID-RECEPTOR COMPLEXES; FISSION YEAST; CYCLOSPORINE-A; TYROSINE PHOSPHORYLATION; GLUCOCORTICOID RECEPTOR; PROLYL ISOMERASE; BINDING-PROTEIN; MITOTIC INDUCER;
D O I
10.1002/j.1460-2075.1996.tb00377.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cyclophilins are peptidyl-prolyl cis-trans isomerases (PPIases) which have been implicated in intracellular protein folding, transport and assembly. Cyclophilins are also known as the intracellular receptors for the immunosuppressive drug cyclosporin A (CsA), The most common type of cyclophilins are the 18 kDa cytosolic proteins containing only the highly conserved core domain for PPIase and CsA binding activities, The wis2(+) gene of the fission yeast Schizosaccharomyces pombe was isolated as a multicopy suppressor of wee1-50 cdc25-22 win1-1, a triple mutant strain which exhibits a cell cycle defect phenotype. Sequence analysis of wis2(+) reveals that it encodes a 40 kDa cyclophilin-like protein, homologous to the mammalian cyclophilin 40. The 18 kDa cyclophilin domain (CyP-18) of wis2 is followed by a C-terminal region of 188 amino acids, The C-terminal region of wis2 is essential for suppression of the triple mutant defect, Furthermore, this region of the protein is able to confer suppression activity on the 18 kDa S.pombe cyclophilin, cyp1, since a hybrid protein consisting of an 18 kDa S.pombe cyclophilin (cyp1) fused to the C-terminus of wis2 shows suppression activity, We also demonstrate that the level of wis2(+) mRNA increases 10- to 20-fold upon heat shock of S.pombe cells, suggesting a role for wis2(+) in the heat-shock response.
引用
收藏
页码:447 / 456
页数:10
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