Heterotrophic cultivation of Euglena gracilis Z for efficient production of α-tocopherol

被引:85
作者
Ogbonna, JC [1 ]
Tomiyama, S [1 ]
Tanaka, H [1 ]
机构
[1] Univ Tsukuba, Inst Appl Biochem, Tsukuba, Ibaraki 305, Japan
关键词
alpha-tocopherol; heterotrophic culture; Euglena gracilis; hydrodynamic stress; dissolved oxygen concentration;
D O I
10.1023/A:1008011201437
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Effects of hydrodynamic stress, dissolved oxygen (DO) concentration and carbon sources on heterotrophic alpha-tocopherol production by Euglena gracilis were investigated. In ajar fermenter without baffle plates, increasing the agitation speed up to 500 rpm had no significant effect on cell growth and alpha-tocopherol production. However, in a jar fermenter equipped with baffle plates, both the cell growth and alpha-tocopherol production were highly suppressed at 500 rpm. At high hydrodynamic stress, the cells secreted nucleic acid-related substances to the culture broth and the shape of the cells shifted from elongated toward spherical. High DO concentration had adverse effects on both cell growth and alpha-tocopherol production, the optimum DO concentration being below 0.8 ppm. In comparison with glucose, the growth rate was lower but the alpha-tocopherol content of the cells was almost four times higher when ethanol was used as the organic carbon source. In a fed-batch culture with ethanol, a very high cell concentration of 39.5 g L-1 was obtained with alpha-tocopherol content of 1200 mu g g-cell(-1). This alpha-tocopherol content is very close to the values reported for photoautotrophic and photoheterotrophic cultures. A very high alpha-tocopherol productivity of 102 mu g L-1 h(-1) was obtained, indicating that heterotrophic cultivation of E. gracilis has a very high potential as a substitute for the current method of extraction from vegetable oils.
引用
收藏
页码:67 / 74
页数:8
相关论文
共 27 条
[1]   DEATH MECHANISMS OF ANIMAL-CELLS IN CONDITIONS OF INTENSIVE AGITATION [J].
ALRUBEAI, M ;
SINGH, RP ;
GOLDMAN, MH ;
EMERY, AN .
BIOTECHNOLOGY AND BIOENGINEERING, 1995, 45 (06) :463-472
[2]  
[Anonymous], 1991, Int. Chem. Eng
[3]   STUDIES OF CHLOROPLAST DEVELOPMENT IN EUGLENA .10. RETURN OF CHLOROPLAST TO PROPLASTID CONDITION DURING DARK ADAPTATION [J].
BENSHAUL, Y ;
EPSTEIN, HT ;
SCHIFF, JA .
CANADIAN JOURNAL OF BOTANY, 1965, 43 (01) :129-&
[4]   TERPENOID METABOLISM IN PLASTIDS - LOCALIZATION OF ALPHA-TOCOPHEROL SYNTHESIS IN CAPSICUM CHROMOPLASTS [J].
CAMARA, B ;
BARDAT, F ;
SEYE, A ;
DHARLINGUE, A ;
MONEGER, R .
PLANT PHYSIOLOGY, 1982, 70 (05) :1562-1563
[5]   SUBSTRATE-INHIBITION OF CHLAMYDOMONAS-REINHARDTII BY ACETATE IN HETEROTROPHIC CULTURE [J].
CHEN, F ;
JOHNS, MR .
PROCESS BIOCHEMISTRY, 1994, 29 (04) :245-252
[6]   Heterotrophic growth of Chlamydomonas reinhardtii on acetate in chemostat culture [J].
Chen, F ;
Johns, MR .
PROCESS BIOCHEMISTRY, 1996, 31 (06) :601-604
[7]  
EHARA T, 1975, J ELECTRON MICROSC, V24, P253
[8]  
ENDO H, 1977, J FERMENT TECHNOL, V55, P369
[9]   INTRACELLULAR-LOCALIZATION OF TOCOPHEROL BIOSYNTHESIS IN CALENDULA-OFFICINALIS [J].
JANISZOWSKA, W .
PHYTOCHEMISTRY, 1987, 26 (05) :1403-1407
[10]  
MIYATAKE K, 1986, IDEN, V40, P49