Application of non-aqueous micellar electrokinetic chromatography to the analysis of active components in radix Salviae miltiorrhizae and its medicinal preparations

被引:33
作者
Chen, AJ
Li, CH
Gao, WH
Hu, ZD [1 ]
Chen, XG
机构
[1] Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China
[2] Shanxi Agr Univ, Taigu 030801, Peoples R China
关键词
tanshinones; non-aqueous micellar electrokinetic chromatography; sodium cholate; second-order derivative electropherograms;
D O I
10.1016/j.jpba.2004.11.040
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A simple, economical and effective non-aqueous micellar electrokinetic chromatography (NAMEKC) method was developed for simultaneous assay of three bioactive components (cryptotanshinone, tanshinone IIA and tanshinone I) in radix Salviae miltiorrhizae and its medicinal preparations for the first time. After optimization of separation conditions, a buffer of 140 mmol l(-1) sodium cholate (SC) in methanol was selected for the separation of the three tanshinones, but baseline separation of tanshinone I and tanshinone IIA in practical samples was not achieved. Therefore, second-order derivative electropherograms were applied for resolving overlapping peaks. Regression equations revealed good linear relationships (correlation coefficients 0.995-0.999) between peak heights in second-order derivative electropherograms and concentrations of the three analytes. The recoveries of three constituents ranged from 91.3 to 105.7%. The results indicated that baseline separation of the analytes was hard to be achieved in practical samples sometimes and second-order derivative electropherograms was applicable for the resolving and analysis of overlapping peaks. (c) 2004 Elsevier B.V. All rights reserved.
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页码:811 / 816
页数:6
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