Rapid movements of vimentin on microtubule tracks: Kinesin-dependent assembly of intermediate filament networks

被引:271
作者
Prahlad, V
Yoon, M
Moir, RD
Vale, RD
Goldman, RD
机构
[1] Northwestern Univ, Sch Med, Dept Cell & Mol Biol, Chicago, IL 60611 USA
[2] Univ Calif San Francisco, Howard Hughes Med Inst, San Francisco, CA 94143 USA
关键词
intermediate filaments; vimentin; microtubule; kinesin; cytoskeleton;
D O I
10.1083/jcb.143.1.159
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The assembly and maintenance of an extended intermediate filament (TF) network in fibroblasts requires microtubule (MT) integrity. Using a green fluorescent protein-vimentin construct, and spreading BHK-21 cells as a model system to study IF-MT interactions, we have discovered a novel mechanism involved in the assembly of the vimentin IF cytoskeleton. This entails the rapid, discontinuous, and MT-dependent movement of IF precursors towards the peripheral regions of the cytoplasm where they appear to assemble into short fibrils. These precursors, or vimentin dots, move at speeds averaging 0.55 +/- 0.24 mu m/s. The vimentin dots colocalize with MT and their motility is inhibited after treatment with nocodazole. Our studies further implicate a conventional kinesin in the movement of the vimentin dots. The dots colocalize with conventional kinesin as shown by indirect immunofluorescence, and IF preparations from spreading cells are enriched in kinesin. Furthermore, microinjection of kinesin antibodies into spreading cells prevents the assembly of an extended IF network. These studies provide insights into the interactions between the IF and MI systems. They also suggest a role for conventional kinesin in the distribution of non-membranous protein cargo, and the local regulation of LF assembly.
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页码:159 / 170
页数:12
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