Crystallographic studies on the ribosome, a large macromolecular assembly exhibiting severe nonisomorphism, extreme beam sensitivity and no internal symmetry

被引:66
作者
Yonath, A [1 ]
Harms, J
Hansen, HAS
Bashan, A
Schlünzen, F
Levin, I
Koelln, I
Tocilj, A
Agmon, I
Peretz, M
Bartels, H
Bennett, WS
Krumbholz, S
Janell, D
Weinstein, S
Auerbach, T
Avila, H
Piolleti, M
Morlang, S
Franceschi, F
机构
[1] Max Planck Unit Ribosomal Struct, Hamburg, Germany
[2] Weizmann Inst Sci, Dept Struct Biol, Rehovot, Israel
[3] Max Planck Inst Mol Genet, Berlin, Germany
来源
ACTA CRYSTALLOGRAPHICA SECTION A | 1998年 / 54卷
关键词
D O I
10.1107/S010876739800991X
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Crystals, diffracting best to around 3 Angstrom, have been grown from intact large and small ribosomal subunits. The bright synchrotron radiation necessary for the collection of the higher-resolution X-ray diffraction data introduces significant decay even at cryo temperatures. Nevertheless, owing to the reasonable isomorphism of the recently improved crystals of the small ribosomal subunits, reliable phases have been extracted at medium resolution (5-6 Angstrom) and an interpretable five-derivative MIR map has been constructed. For the crystals of the large subunits, however, the situation is more complicated because at higher resolution (2.7-7 Angstrom) they suffer from substantial radiation sensitivity, a low level of isomorphism, instability of the longest unit-cell axis and nonisotropic mosaicity. The 8 Angstrom MIR map, constructed to gain insight into this unusual system, may provide feasible reasoning for the odd combination of the properties of these crystals as well as hints for future improvement. Parallel efforts, in which electron-microscopy-reconstructed images are being exploited for molecular-replacement studies, are also discussed.
引用
收藏
页码:945 / 955
页数:11
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