Assessment of mycobacterial DNA in cells and tissues of mycobacterial and sarcoid lesions

被引：65

作者：

Righter, E

Greinert, U

Kirsten, D

RuschGerdes, S

Schluter, G

Duchrow, M

Galle, J

Magnussen, H

Schlaak, M

Flad, HD

Gerdes, J

机构：

[1] FORSCHUNGSINST BORSTEL,DEPT CLIN MED,D-23845 BORSTEL,GERMANY

[2] FORSCHUNGSINST BORSTEL,DEPT IMMUNOL & CELL BIOL,W-2061 BORSTEL,GERMANY

[3] KRANKENHAUS GROSSHANSDORF,W-2070 GROSSHANSDORF,GERMANY

来源：

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE | 1996年 / 153卷 / 01期

关键词：

D O I：

10.1164/ajrccm.153.1.8542146

中图分类号：

R4 [临床医学];

学科分类号：

1002 ; 100602 ;

摘要：

In this study we applied a polymerase chain reaction (PCR) assay for the detection and species-specific identification of mycobacteria to samples from patients with sarcoidosis and mycobacterial infections and from control patients. The PCR-technique is based on the amplification of mycobacterial DNA coding for 16S rRNA, which is present in all mycobacterial species, and on the additional sequencing of the PCR fragment to determine the species. Mycobacterial DNA could be detected in lung tissues and bronchoalveolar lavage cells from cases of tuberculosis and infections with atypical mycobacteria. On the other hand, mycobacterial DNA was amplified only in lung tissue from one patient with sarcoidosis. Twenty-three samples from patients with sarcoidosis were negative for mycobacterial DNA. From our results we conclude that the granulomatous lesions in sarcoidosis may not be due to mycobacterial infections.

引用

页码：375 / 380

页数：6

共 23 条

[1] CHARACTERIZATION OF A DNA PROBE FOR DETECTION OF MYCOBACTERIUM-TUBERCULOSIS COMPLEX IN CLINICAL-SAMPLES BY POLYMERASE CHAIN-REACTION [J].

ALTAMIRANO, M ;

KELLY, MT ;

WONG, A ;

BESSUILLE, ET ;

BLACK, WA ;

SMITH, JA .

JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (08) :2173-2176

[2] A SEARCH FOR MYCOBACTERIAL DNA IN GRANULOMATOUS TISSUES FROM PATIENTS WITH SARCOIDOSIS USING THE POLYMERASE CHAIN-REACTION [J].

BOCART, D ;

LECOSSIER, D ;

DELASSENCE, A ;

VALEYRE, D ;

BATTESTI, JP ;

HANCE, AJ .

AMERICAN REVIEW OF RESPIRATORY DISEASE, 1992, 145 (05) :1142-1148

[3]

BODDINGHAUS B, 1990, J CLIN MICROBIOL, V28, P1751

[4] DIAGNOSIS OF TUBERCULOSIS BY DNA AMPLIFICATION IN CLINICAL-PRACTICE EVALUATION [J].

BRISSONNOEL, A ;

AZNAR, C ;

CHUREAU, C ;

NGUYEN, S ;

PIERRE, C ;

BARTOLI, M ;

BONETE, R ;

PIALOUX, G ;

GICQUEL, B ;

GARRIGUE, G .

LANCET, 1991, 338 (8763) :364-366

[5] MYCOBACTERIUM-CELATUM SP-NOV [J].

BUTLER, WR ;

OCONNOR, SP ;

YAKRUS, MA ;

SMITHWICK, RW ;

PLIKAYTIS, BB ;

MOSS, CW ;

FLOYD, MM ;

WOODLEY, CL ;

KILBURN, JO ;

VADNEY, FS ;

GROSS, WM .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1993, 43 (03) :539-548

[6] THE 16S RIBOSOMAL-RNA OF MYCOBACTERIUM-LEPRAE CONTAINS A UNIQUE SEQUENCE WHICH CAN BE USED FOR IDENTIFICATION BY THE POLYMERASE CHAIN-REACTION [J].

COX, RA ;

KEMPSELL, K ;

FAIRCLOUGH, L ;

COLSTON, MJ .

JOURNAL OF MEDICAL MICROBIOLOGY, 1991, 35 (05) :284-290

[7]

Davis LG., 1986, Basic Methods in Molecular Biology

[8] ISOLATION AND DIRECT COMPLETE NUCLEOTIDE DETERMINATION OF ENTIRE GENES - CHARACTERIZATION OF A GENE CODING FOR 16S-RIBOSOMAL RNA [J].

EDWARDS, U ;

ROGALL, T ;

BLOCKER, H ;

EMDE, M ;

BOTTGER, EC .

NUCLEIC ACIDS RESEARCH, 1989, 17 (19) :7843-7853

[9] MYCOBACTERIUM-TUBERCULOSIS DNA IN TISSUE AFFECTED BY SARCOIDOSIS [J].

FIDLER, HM ;

ROOK, GA ;

JOHNSON, NM ;

MCFADDEN, J .

BRITISH MEDICAL JOURNAL, 1993, 306 (6877) :546-549

[10] MYCOBACTERIAL NUCLEIC-ACIDS IN SARCOID LESIONS [J].

GERDES, J ;

RICHTER, E ;

RUSCHGERDES, S ;

GREINERT, U ;

GALLE, J ;

SCHLAAK, M ;

FLAD, HD ;

KIRSTEN, D ;

MAGNUSSEN, H .

LANCET, 1992, 339 (8808) :1536-1537

← 1 2 3 →