The Mouse Lymphoma Assay

被引：60

作者：

Clements, J ^{[1
]}

机构：

[1] Covance Labs Ltd, Harrogate HG3 1PY, N Yorkshire, England

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 2000年 / 455卷 / 1-2期

关键词：

Mouse Lymphoma Assay; microwell; soft agar; cytotoxicity; 24-h treatments;

D O I：

10.1016/S0027-5107(00)00066-X

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this paper, the current status of the protocol for the Mouse Lymphoma Assay is discussed. A brief history describes the events leading to current protocol recommendations. Areas for further development such as cytotoxicity, 24-h treatments, acceptability criteria and statistical analysis are also considered. Recent guidelines are reviewed, and consensus issues from the Mouse Lymphoma workgroup assembled as part of the International Workshop on Genotoxicity Test Procedures (IWGTP) are included. There are two versions of the assay - soft agar and microwell - and both will be discussed. For assay procedures, the emphasis will be on a typical microwell protocol but an attempt will be made to highlight protocol variations between laboratories and between the microwell and agar versions of the assay. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：97 / 110

页数：14

共 39 条

[1]

[Anonymous], 1990, STAT EVALUATION MUTA

[2]

[Anonymous], 1997, 476 OECD

[3]

[Anonymous], 1995, INT C HARM TECHN REQ

[4] MOLECULAR DISSECTION OF MUTATIONS AT THE HETEROZYGOUS THYMIDINE KINASE LOCUS IN MOUSE LYMPHOMA-CELLS [J].

APPLEGATE, ML ;

MOORE, MM ;

BRODER, CB ;

BURRELL, A ;

JUHN, G ;

KASWECK, KL ;

LIN, PF ;

WADHAMS, A ;

HOZIER, JC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (01) :51-55

[5] CHROMOSOME ANALYSIS OF SMALL AND LARGE L5178Y MOUSE LYMPHOMA CELL COLONIES - COMPARISON OF TRIFLUOROTHYMIDINE-RESISTANT AND UNSELECTED CELL COLONIES FROM MUTAGEN-TREATED AND CONTROL CULTURES [J].

BLAZAK, WF ;

LOS, FJ ;

RUDD, CJ ;

CASPARY, WJ .

MUTATION RESEARCH, 1989, 224 (02) :197-208

[6] GENOTOXIC EFFECTS IN CULTURED-MAMMALIAN-CELLS PRODUCED BY LOW PH TREATMENT CONDITIONS AND INCREASED ION CONCENTRATIONS [J].

BRUSICK, D .

ENVIRONMENTAL MUTAGENESIS, 1986, 8 (06) :879-886

[7] Identification and chromosomal assignment of two heterozygous mutations in the Trp53 gene in L5178Y/Tk±-3.7.2C mouse lymphoma cells [J].

Clark, LS ;

Hart, DW ;

Vojta, PJ ;

Harrington-Brock, K ;

Barrett, JC ;

Moore, MM ;

Tindall, KR .

MUTAGENESIS, 1998, 13 (05) :427-434

[8]

Clements J., 1998, Environmental and Molecular Mutagenesis, V31, P45

[9]

CLEMENTS J, 1997, MUTAT RES S1, V379, P194

[10] GUIDE FOR PERFORMING THE MOUSE LYMPHOMA ASSAY FOR MAMMALIAN-CELL MUTAGENICITY [J].

CLIVE, D ;

CASPARY, W ;

KIRBY, PE ;

KREHL, R ;

MOORE, M ;

MAYO, J ;

OBERLY, TJ .

MUTATION RESEARCH, 1987, 189 (02) :143-156

← 1 2 3 4 →