A small peptide derived from Flt-1 (VEGFR-1) functions as an angiogenic inhibitor

被引:35
作者
Tan, DCW
Kini, RM
Jois, SDS
Lim, DKF
Xin, LX
Ge, RW [1 ]
机构
[1] Natl Univ Singapore, Dept Sci Biol, Singapore 119260, Singapore
[2] Natl Univ Singapore, Dept Pharm, Singapore 119260, Singapore
关键词
vascular endothelial growth factor (vascular; permeability factor); Flt-1(VEGFR-1); angiogenesis; Miles' assay; chick chorioallantoic membrane;
D O I
10.1016/S0014-5793(01)02314-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Vascular endothelial growth factor (VEGF) is an angiogenic stimulator which functions through two endothelial specific tyrosine kinase receptors, Flt-1 and Flk-1, In this work, we show that an 11-amino acid peptide derived from the second immunoglobulin-like domain of Flt-1 functions as an angiogenic inhibitor in chick chorioallantoic membrane and inhibited VEGF-induced vascular permeability in Miles' assay without binding to VEGF directly, Circular dichroism and nuclear magnetic resonance analyses indicate that this peptide forms a stable extended structure in solution, presumably beta -sheet structure and is most likely existing as a dimer, Our results suggest that this small peptide functions as an angiogenic inhibitor by inhibiting VEGF function through a non-VEGF binding mechanism, (C) 2001 Federation of European Biochemical Societies, Published by Elsevier Science B,V, All rights reserved.
引用
收藏
页码:150 / 156
页数:7
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