The mode of inhibitory action of alpha-mangostin, a novel inhibitor, on the sarcoplasmic reticulum Ca2+-pumping ATPase from rabbit skeletal muscle

被引：13

作者：

Furukawa, K ^{[1
]}

Shibusawa, K ^{[1
]}

Chairungsrilerd, N ^{[1
]}

Ohta, T ^{[1
]}

Nozoe, S ^{[1
]}

Ohizumi, Y ^{[1
]}

机构：

[1] TOHOKU UNIV,FAC PHARMACEUT SCI,DEPT PHARMACOGNOSY,AOBA KU,SENDAI,MIYAGI 980,JAPAN

来源：

JAPANESE JOURNAL OF PHARMACOLOGY | 1996年 / 71卷 / 04期

关键词：

alpha-mangostin; Ca2+-pumping ATPase; sarcoplasmic reticulum;

D O I：

10.1254/jjp.71.337

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

alpha-Mangostin, the principal ingredient of the fruit hull of Garcinia mangostana, caused a concentration-dependent decrease in the activities of both Ca2+-ATPase and Ca2+-transport of the sarcoplasmic reticulum from rabbit skeletal muscle with an IC50 value of 5 mu M. Neither Ca2+ release nor other enzyme activities were affected by alpha-mangostin. Kinetic analysis of the inhibitory effects of alpha-mangostin on Ca2+-ATPase suggests that the inhibition of the ATPase is a noncompetitive-type with respect to ATP or Ca2+. alpha-Mangostin may become a useful pharmacological tool for clarifying the physiological functions of Ca2+-pumping ATPase and sarcoplasmic reticulum.

引用

页码：337 / 340

页数：4

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