Serodiagnosis of cat scratch disease:: Response to Bartonella henselae in children and a review of diagnostic methods

被引:83
作者
Sander, A [1 ]
Berner, R
Ruess, M
机构
[1] Univ Freiburg, Inst Med Microbiol & Hyg, D-79104 Freiburg, Germany
[2] Univ Freiburg, Childrens Hosp, D-79104 Freiburg, Germany
关键词
D O I
10.1007/PL00011280
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
In this study, sera from 116 children without clinical evidence of cat scratch disease (CSD) and sera from 19 children with CSD were investigated by two different immunofluorescent assays (test A and test B) to evaluate the seroprevalence of Bartonella henselae antibodies in this population. Antibodies against Bartonella henselae were found in noninfected children only in low titers: 13% and 3.5% of serum samples were positive by test A and test B, respectively. Ige titers as deter mined by test A ranged from 1:64 to 1:256, whereas test B did not yield titers > 1:64. Of the 19 children with CSD, 7 (37%) and 5 (26%) had low antibody titers (1:64-1:256) on admission by test A and test B, respectively. In such cases, IgM antibodies against Bartonella henselae and/or a titer increase in the following weeks are required to prove suspected CSD. The results indicate that the seroprevalence of Bartonella henselae antibodies in children without evidence of CSD is low, and that the results may differ depending on the tests being used. Low antibody titers, however, were found not only in healthy children but also among patients with CSD, which could be indicative of the beginning or of the end of an illness. Currently, only few data are available concerning the seroprevalence of antibodies to Bartonella spp. among children. Although children are often affected by CSD, the specificity of most tests has been evaluated by investigating blood donors, who are usually adults. Several different serological tests are used for the diagnosis of CSD, including the immunofluorescence assay, the enzyme-linked immunosorbent assay, and Western blot (WB) analysis. The sensitivities of different IFAs range from 14 to 100%, depending on the antigen used, the cut-off chosen, and the test procedures. The current diagnostic value of different serological tests for diagnosis of CSD is reviewed.
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页码:392 / 401
页数:10
相关论文
共 49 条
[1]   Diagnostic value of the indirect immunofluorescence assay in cat scratch disease with Bartonella henselae and Afipia felis antigens [J].
Amerein, MP ;
DeBriel, D ;
Jaulhac, B ;
Meyer, P ;
Monteil, H ;
Piemont, Y .
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 1996, 3 (02) :200-204
[2]   CHARACTERIZATION OF A 17-KILODALTON ANTIGEN OF BARTONELLA-HENSELAE REACTIVE WITH SERA FROM PATIENTS WITH CAT-SCRATCH DISEASE [J].
ANDERSON, B ;
LU, E ;
JONES, D ;
REGNERY, R .
JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (09) :2358-2365
[3]   EIA FOR DETECTION OF ROCHALIMAEA-HENSELAE - REACTIVE IGG, IGM, AND IGA ANTIBODIES IN PATIENTS WITH SUSPECTED CAT-SCRATCH DISEASE [J].
BARKA, NE ;
HADFIELD, T ;
PATNAIK, M ;
SCHWARTZMAN, WA ;
PETER, JB .
JOURNAL OF INFECTIOUS DISEASES, 1993, 167 (06) :1503-1504
[4]   Prospective randomized double blind placebo-controlled evaluation of azithromycin for treatment of cat-scratch disease [J].
Bass, JW ;
Freitas, BC ;
Freitas, AD ;
Freitas, D ;
Sisler, CL ;
Chan, DS ;
Vincent, JM ;
Person, DA ;
Claybaugh, JR ;
Wittler, RR ;
Weisse, ME ;
Regnery, RL ;
Slater, LN .
PEDIATRIC INFECTIOUS DISEASE JOURNAL, 1998, 17 (06) :447-452
[5]   BARTONELLA-HENSELAE AND BARTONELLA-QUINTANA ADHERENCE TO AND ENTRY INTO CULTURED HUMAN EPITHELIAL-CELLS [J].
BATTERMAN, HJ ;
PEEK, JA ;
LOUTIT, JS ;
FALKOW, S ;
TOMPKINS, LS .
INFECTION AND IMMUNITY, 1995, 63 (11) :4553-4556
[6]   Molecular analysis of riboflavin synthesis genes in Bartonella henselae and use of the ribC gene for differentiation of Bartonella species by PCR [J].
Bereswill, S ;
Hinkelmann, S ;
Kist, M ;
Sander, A .
JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (10) :3159-3166
[7]   Pitfalls and fallacies of cat scratch disease serology: Evaluation of Bartonella henselae-based indirect fluorescence assay and enzyme-linked immunoassay [J].
Bergmans, AMC ;
Peeters, MF ;
Schellekens, JFP ;
Vos, MC ;
Sabbe, LJM ;
Ossewaarde, JM ;
Verbakel, H ;
Hooft, HJ ;
Schouls, LM .
JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (08) :1931-1937
[8]   Predominance of two Bartonella henselae variants among cat-scratch disease patients in the Netherlands [J].
Bergmans, AMC ;
Schellekens, JFP ;
vanEmbden, JDA ;
Schouls, LM .
JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (02) :254-260
[9]  
BOX ATA, 1996, J MICROBIOL METH, V27, P101
[10]  
CIMOLAI N, 1999, J MICROBIOLOGICAL ME, V37, P276