Construction of a plasminogen activator inhibitor-1 variant without measurable affinity to vitronectin but otherwise normal

被引：25

作者：

Jensen, JK ^{[1
]}

Durand, MKV ^{[1
]}

Skeldal, S ^{[1
]}

Dupont, DM ^{[1
]}

Bodker, JS ^{[1
]}

Wind, T ^{[1
]}

Andreasen, PA ^{[1
]}

机构：

[1] Aarhus Univ, Dept Mol Biol, Lab Cellular Prot Sci, DK-8000 Aarhus C, Denmark

来源：

FEBS LETTERS | 2004年 / 556卷 / 1-3期

关键词：

plasminogen activator inhibitor-1; plasminogen; serpin; vitronectin; adhesion;

D O I：

10.1016/S0014-5793(03)01405-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Vitronectin (VN) and plasminogen activator inhibitor-1 (PAI-1) have important functional interactions: VN stabilises the protease inhibitory activity of PAI-1 and PAI-1 inhibits binding of adhesion receptors to VN. Having previously mapped the PAI-1 binding area for VN, we have now constructed a PAI-1 variant, R103A-M112A-Q125A, without measurable affinity to VN, but with full protease inhibitory activity and endocytosis receptor binding. As a tool for evaluating the physiological and pathophysiological functions of the PAI-1-VN interaction, our new variant is far superior to the previously widely used PAI-1 variant Q125K, which we have found possesses an only about 10-fold reduced affinity to VN. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：175 / 179

页数：5

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