Leishmania (Viannia) braziliensis is the predominant species infecting patients with American cutaneous leishmaniasis in the State of Minas Gerais, Southeast Brazil

被引：56

作者：

Passos, VMA

Fernandes, O

Lacerda, PAF

Volpini, AC

Gontijo, CMF

Degrave, W

Romanha, AJ ^{[1
]}

机构：

[1] FIOCRUZ, Ctr Pesquisas Rene Rachou, BR-30190002 Belo Horizonte, MG, Brazil

[2] Univ Fed Minas Gerais, Fac Med, Dept Clin Med, BR-0130100 Belo Horizonte, MG, Brazil

[3] FIOCRUZ, Inst Oswaldo Cruz, Dept Bioquim & Biol Mol, Belo Horizonte, MG, Brazil

来源：

ACTA TROPICA | 1999年 / 72卷 / 03期

关键词：

American cutaneous leishmaniasis; human infection; Brazil; molecular characterization of parasites; Leishmania (V.) braziliensis;

D O I：

10.1016/S0001-706X(98)00100-4

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Skin biopsies from 53 patients with American cutaneous leishmaniasis (ACL) from the State of Minas Gerais, Brazil, were used for a characterization of the Leishmania parasites. A pair of primers flanking the conserved region of the Leishmania minicircle kDNA was used to obtain amplified DNA via the polymerase chain reaction. The amplified products were subsequently hybridized with Leishmania subgenus-specific radiolabeled probes. Parasites from 49 out of 53 samples (92.5%) were characterized as belonging to the subgenus Viannia and four (7.5%) as belonging to the subgenus Leishmania. Clinical, epidemiological and molecular evidence allow us to conclude that Leishmania (V.) braziliensis and Leishmania (L.) amazonensis are the species present in the patients studied and that L. (V.) braziliensis is the predominant species in the State of Minas Gerais, Brazil. (C) 1999 Published by Elsevier Science B.V. All rights reserved.

引用

页码：251 / 258

页数：8

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