Prevention of cross-contamination during sampling procedure in molecular detection for cancer micrometastasis

被引：7

作者：

Fujiwara, Y ^{[1
]}

Ooka, M ^{[1
]}

Sugita, Y ^{[1
]}

Sakita, I ^{[1
]}

Tamaki, Y ^{[1
]}

Monden, M ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Med, Dept Surg & Clin Oncol, Suita, Osaka 5650871, Japan

来源：

CANCER LETTERS | 2000年 / 153卷 / 1-2期

关键词：

micrometastasis; reverse transcriptase-polymerase chain reaction; cross-contamination; lymph node; breast cancer;

D O I：

10.1016/S0304-3835(00)00355-4

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Reverse transcriptase-polymerase chain reaction (RT-PCR) techniques have been widely employed as an ultra-sensitive method for detection of micrometastases in patients with various types of malignancies. Messenger RNA of a specific marker gene is a target for RT-PCR amplification to examine the presence of micrometastases in body fluids or tissues obtained from human. We developed the RT-PCR assay specific for rat p-actin mRNA, which cannot detect human counterpart and assessed how much contamination of rat tissues can influence the result of RT-PCR assay and how to avoid the influence of the contamination in RT-PCR assay. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：109 / 111

页数：3