Rapid PCR-based method for the direct analysis of fungal communities in complex environmental samples

被引：48

作者：

Pennanen, T ^{[1
]}

Paavolainen, L ^{[1
]}

Hantula, J ^{[1
]}

机构：

[1] Finnish Forest Res Inst, Vantaa 01301, Finland

来源：

SOIL BIOLOGY & BIOCHEMISTRY | 2001年 / 33卷 / 4-5期

基金：

芬兰科学院;

关键词：

18S rRNA; fungi; DGGE; Betula pendula; forest soil;

D O I：

10.1016/S0038-0717(00)00198-X

中图分类号：

S15 [土壤学];

学科分类号：

0903 ; 090301 ;

摘要：

Fungal community analysis using 18S rDNA primer pairs and denaturing gradient gel electrophoresis of PCR products (Vainio, E.J.. Hantula, J., 2000. Mycological Research 104, 927-936) was applied to field studies of the forest ecosystem. We report a DNA extraction method producing high quality DNA allowing successful PCR amplification from problematic samples without use of nested polymerase chain reaction (PCR) procedures. The analysis was found to be applicable for samples from environments of varying fungal diversities and high organic matter content: wood samples from fallen branches of trees, laboratory mini-ecosystems and forest humus samples. When the method was tested using replicate forest soil samples, it was shown to be highly reproducible. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：697 / 699

页数：3

共 6 条

[1] Detection and characterization of fungal infections of Ammophila arenaria (marram grass) roots by denaturing gradient gel electrophoresis of specifically amplified 18S rDNA [J].