Development of a biosensor for glycated hemoglobin

被引:36
作者
Halámek, J. [1 ]
Wollenberger, U. [1 ]
Stoecklein, W. [1 ]
Scheller, F. W. [1 ]
机构
[1] Univ Potsdam, Inst Biochem & Biol, Dept Analyt Biochem, D-14476 Potsdam, Germany
关键词
EQCM; glycated hemoglobin; boronic acid; ferrocene; chemically modified electrode;
D O I
10.1016/j.electacta.2007.03.059
中图分类号
O646 [电化学、电解、磁化学];
学科分类号
081704 ;
摘要
The development of an electrochemical piezoelectric sensor for the detection of glycated hemoglobin is presented. The total hemoglobin (Hb) content is monitored with a mass-sensitive quartz crystal modified with surfactants, and the glycated fraction of the immobilized Hb is determined by subsequent voltarnmetric measurement of the coupled ferroceneboronic acid. Different modifications of the sensor were tested for their hemoglobin binding ability. Deoxycholate (DOCA) was found to be the most suitable among the examined modifiers. Piezoelectric quartz crystals with gold electrodes were modified with DOCA by covalent binding to a pre-formatted 4-aminothiophenol monolayer. The properties of the Hb binding to DOCA and the pH effect on this interaction were studied. In the proposed assay for glycated hemoglobin at first an Hb sample is incubated with ferroceneboronic acid (FcBA), which binds to the fructosyl residue of the glycated Hb. Then this preincubated Hb sample is allowed to interact with the DOCA-modified piezoelectric quartz crystal. The binding is monitored by quartz crystal nanobalance QCN). The amount of FcBA present on the sensor surface is determined by square wave voltammetry. The binding of FcBA results in well-defined peaks with an EO' of +200 mV versus Ag/AgC1 (1 M KC1). The peak height depends on the degree of glycated Hb in the sample ranging from 0% to 20% of total Hb. The regeneration of the sensing surface is achieved by pepsin digestion of the deposited Hb. Thus the sensor can be re-used more than 30 times. (c) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1127 / 1133
页数:7
相关论文
共 27 条
[1]   FURTHER IDENTIFICATION OF NATURE AND LINKAGE OF CARBOHYDRATE IN HEMOGLOBIN-A1C [J].
BUNN, HF ;
HANEY, DN ;
GABBAY, KH ;
GALLOP, PM .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1975, 67 (01) :103-109
[2]   Direct electron transfer between hemoglobin and pyrolytic graphite electrodes enhanced by Fe3O4 nanoparticles in their layer-by-layer self-assembly films [J].
Cao, Dongfang ;
Hu, Naifei .
BIOPHYSICAL CHEMISTRY, 2006, 121 (03) :209-217
[3]   The characterization and bioelectrocatalytic properties of hemoglobin by direct electrochemistry of DDAB film modified electrodes [J].
Chen, SM ;
Tseng, CC .
ELECTROCHIMICA ACTA, 2004, 49 (12) :1903-1914
[4]   Ordered electrochemically active films of hemoglobin, didodecyldimethylammonium ions, and clay [J].
Chen, XL ;
Hu, NF ;
Zeng, YH ;
Rusling, JF ;
Yang, J .
LANGMUIR, 1999, 15 (20) :7022-7030
[5]   Binding of chlorpyrifos and cypermethrin to blood proteins [J].
Cui, Y ;
Guo, JF ;
Xu, BJ ;
Chen, ZY .
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 2006, 85 (02) :110-114
[6]  
DRABKIN DL, 1946, J BIOL CHEM, V164, P703
[7]   INVITRO SYNTHESIS OF HEMOGLOBIN AIC [J].
FLUCKIGER, R ;
WINTERHALTER, KH .
FEBS LETTERS, 1976, 71 (02) :356-360
[8]   Characterization of binding of cholinesterases to surface immobilized ligands [J].
Halamek, Jan ;
Teller, Carsten ;
Zeravik, Jiri ;
Fournier, Didier ;
Makower, Alexander ;
Scheller, Frieder W. .
ANALYTICAL LETTERS, 2006, 39 (08) :1491-1502
[9]   Driving forces for layer-by-layer self-assembly of films of SiO2 nanoparticles and heme proteins [J].
He, PL ;
Hu, NF ;
Rusling, JF .
LANGMUIR, 2004, 20 (03) :722-729
[10]   Assembly of electroactive layer-by-layer films of hemoglobin and polycationic poly(diallyldimethylammonium) [J].
He, PL ;
Hu, NF ;
Zhou, G .
BIOMACROMOLECULES, 2002, 3 (01) :139-146