Cell type and pathway dependence of synaptic AMPA receptor number and variability in the hippocampus

被引：633

作者：

Nusser, Z ^{[1
]}

Lujan, R ^{[1
]}

Laube, G ^{[1
]}

Roberts, JDB ^{[1
]}

Molnar, E ^{[1
]}

Somogyi, P ^{[1
]}

机构：

[1] Univ Oxford, MRC, Anat Neuropharmacol Unit, Dept Pharmacol, Oxford OX1 3TH, England

来源：

NEURON | 1998年 / 21卷 / 03期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/S0896-6273(00)80565-6

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

It has been suggested that some glutamatergic synapses lack functional AMPA receptors. We used quantitative immunogold localization to determine the number and variability of synaptic AMPA receptors in the rat hippocampus. Three classes of synapses show distinct patterns of AMPA receptor content. Mossy fiber synapses on CA3 pyramidal spines and synapses on GABAergic interneurons are all immunopositive, have less variability, and contain 4 times as many AMPA receptors as synapses made by Schaffer collaterals on CA1 pyramidal spines and by commissural/associational (C/A) terminals on CA3 pyramidal spines. Up to 17% of synapses in the tatter two connections are immunonegative. After calibrating the immunosignal (1 gold = 2.3 functional receptors) at mossy synapses of a 17-day-old rat, we estimate that the AMPA receptor content of CIA synapses on CA3 pyramidal spines ranges from <3 to 140. A similar range is found in adult Schaffer collateral and C/A synapses.

引用

页码：545 / 559

页数：15

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