Chemokine-induced cell death in CCR5-expressing neuroblastoma cells

被引：22

作者：

Cartier, L

Dubois-Dauphin, M

Hartley, O

Irminger-Finger, I

Krause, KH ^{[1
]}

机构：

[1] Univ Hosp Geneva, Dept Geriatr, Biol Ageing Lab, CH-1225 Chene Bourg, Switzerland

[2] Univ Geneva, Fac Med, Dept Med Biochem, CH-1211 Geneva, Switzerland

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2003年 / 145卷 / 1-2期

基金：

瑞士国家科学基金会;

关键词：

CCR5; neuroblastoma cell line; beta-chemokines; apoptosis; caspase-3; microglia;

D O I：

10.1016/j.jneuroim.2003.09.008

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

CCR5 is expressed in neurons but its function in this cellular context is hitherto poorly understood. We have generated CCR5-expressing SH-SY5Y neuroblastoma cells. CCR5 ligands induced cell death in these cells, but not in control neuroblastoma cells or in CCR5-expressing fibroblasts. CCR5-dependent killing of neuroblastoma cells occurred through apoptosis, since it was accompanied by caspase-3 activation and could be prevented by a caspase-3 inhibitor. Finally, cell killing by activated microglia was more rapid and extensive in CCR5-expressing neuroblastoma cells than in control cells. In summary, CCR5 may act as a death receptor in cells of neuronal lineage and therefore be involved in inflammatory neurodegeneration. (C) 2003 Elsevier B.V All rights reserved.

引用

收藏

页码：27 / 39

页数：13

相关论文

共 83 条

[1] CCR5 mediates Fas- and caspase-8 dependent apoptosis of both uninfected and HIV infected primary human CD4 T cells [J].

Algeciras-Schimnich, A ;

Vlahakis, SR ;

Villasis-Keever, A ;

Gomez, T ;

Heppelmann, CJ ;

Bou, G ;

Paya, CV .

AIDS, 2002, 16 (11) :1467-1478

[2] CC CKRS: A RANTES, MIP-1 alpha, MIP-1 beta receptor as a fusion cofactor for macrophage-tropic HIV-1 [J].

Alkhatib, G ;

Combadiere, C ;

Broder, CC ;

Feng, Y ;

Kennedy, PE ;

Murphy, PM ;

Berger, EA .

SCIENCE, 1996, 272 (5270) :1955-1958

[3] The role of the CD4 receptor versus HIV coreceptors in envelope-mediated apoptosis in peripheral blood mononuclear cells [J].

Arthos, J ;

Cicala, C ;

Selig, SM ;

White, AA ;

Ravindranath, HM ;

Van Ryk, D ;

Steenbeke, TD ;

Machado, E ;

Khazanie, P ;

Hanback, MS ;

Hanback, DB ;

Rabin, RL ;

Fauci, AS .

VIROLOGY, 2002, 292 (01) :98-106

[4] Expression of chemokine receptors in the rat brain [J].

Bajetto, A ;

Bonavia, R ;

Barbero, S ;

Florio, T ;

Costa, A ;

Schettini, G .

NEUROENDOCRINE IMMUNE BASIS OF THE RHEUMATIC DISEASES, 1999, 876 :201-209

[5] Characterization of chemokines and their receptors in the central nervous system: physiopathological implications [J].

Bajetto, A ;

Bonavia, R ;

Barbero, S ;

Schettini, G .

JOURNAL OF NEUROCHEMISTRY, 2002, 82 (06) :1311-1329

[6] CROSS-LINKING CD4 BY HUMAN IMMUNODEFICIENCY VIRUS-GP120 PRIMES T-CELLS FOR ACTIVATION-INDUCED APOPTOSIS [J].

BANDA, NK ;

BERNIER, J ;

KURAHARA, DK ;

KURRLE, R ;

HAIGWOOD, N ;

SEKALY, RP ;

FINKEL, TH .

JOURNAL OF EXPERIMENTAL MEDICINE, 1992, 176 (04) :1099-1106

[7] The HIV coreceptors CXCR4 and CCR5 are differentially expressed and regulated on human T lymphocytes [J].

Bleul, CC ;

Wu, LJ ;

Hoxie, JA ;

Springer, TA ;

Mackay, CR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (05) :1925-1930

[8] Primary sensory neurons migrate in response to the chemokine RANTES [J].

Bolin, LM ;

Murray, R ;

Lukacs, NW ;

Strieter, RM ;

Kunkel, SL ;

Schall, TJ ;

Bacon, KB .

JOURNAL OF NEUROIMMUNOLOGY, 1998, 81 (1-2) :49-57

[9] Cellular expression of functional chemokine receptor CCR5 and CXCR4 in human embryonic neurons [J].

Boutet, A ;

Salim, H ;

Leclerc, P ;

Tardieu, M .

NEUROSCIENCE LETTERS, 2001, 311 (02) :105-108

[10] MODULATION OF HUMAN MICROGLIAL CELL SUPEROXIDE PRODUCTION BY CYTOKINES [J].

CHAO, CC ;

HU, SX ;

PETERSON, PK .

JOURNAL OF LEUKOCYTE BIOLOGY, 1995, 58 (01) :65-70

← 1 2 3 4 5 6 7 8 9 →