An intrinsically protein-resistant surface plasmon resonance biosensor based upon a RF-plasma-deposited thin film

被引：58

作者：

Mar, MN

Ratner, BD

Yee, SS

机构：

[1] Univ Washington, Dept Bioengn, Seattle, WA 98195 USA

[2] Univ Washington, Dept Elect Engn, Seattle, WA 98195 USA

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1999年 / 54卷 / 1-2期

基金：

美国国家卫生研究院;

关键词：

surface plasmon resonance; biosensors; nonfouling surfaces; triethylene glycol dimethyl ether;

D O I：

10.1016/S0925-4005(98)00330-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The development of a nonfouling chemical overlayer for a surface plasmon resonance (SPR) biosensor is presented. Triethylene glycol dimethyl ether was deposited onto SPR substrates from a radio frequency glow discharge plasma reactor. A thin film was produced that is rich in poly(ethylene oxide) groups that are known to resist biomolecule uptake. X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy were utilized to identify and verify film composition. The films are shown to be highly resistant to bovine serum albumin fouling in a planar SPR apparatus. These results indicate the promise of plasma-deposited triglyme to modify substrates previously compromised by biological fouling. (C) 1999 Elsevier Science S.A. Al rights reserved.

引用

页码：125 / 131

页数：7

共 20 条

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