On the Enzymatic Properties of Dnmt1 Specificity, Processivity, Mechanism of Linear Diffusion and Allosteric Regulation of the Enzyme

被引:90
作者
Jeltsch, Albert [1 ]
机构
[1] Int Jacobs Univ Bremen, Sch Sci & Engn, D-28759 Bremen, Germany
关键词
DNA methyltransferase; DNA methylation; Dnmt1; enzyme mechanism; enzyme kinetics; enzyme regulation; maintenance methylation; protein DNA interaction;
D O I
10.4161/epi.1.2.2767
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this short review the enzymatic properties of Dnmt1 are summarized. Studies on the specificity of Dnmt1 have shown that it has 30-40 fold preference for hemimethylated target sites. It methylates hemimethylated DNA in a processive reaction, moving on the DNA in a random walk. Binding of DNA to allosteric site(s) in the N-terminal part of the enzyme can lead to stimulation and inhibition of its catalytic activity depending on the nature of the substrate and effector.
引用
收藏
页码:63 / 66
页数:4
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