Increase in uncoupling protein-2 mRNA expression by BRL49653 and bromopalmitate in human adipocytes

被引：52

作者：

Viguerie-Bascands, N ^{[1
]}

Saulnier-Blache, JS ^{[1
]}

Dandine, M ^{[1
]}

Dauzats, M ^{[1
]}

Daviaud, D ^{[1
]}

Langin, D ^{[1
]}

机构：

[1] Univ Toulouse 3, Rangueil Hosp, INSERM, U317,Louis Bugnard Inst, F-31403 Toulouse 4, France

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1999年 / 256卷 / 01期

关键词：

D O I：

10.1006/bbrc.1999.0303

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Uncoupling protein-2 (UCP2) is a novel mitochondrial protein that may be involved in the control of energy expenditure. We have previously reported an upregulation of adipose tissue UCP2 mRNA expression during fasting in humans. Analysis of changes in metabolic parameters suggested that fatty acids may be associated with the increased UCP2 mRNA level. Culture of human adipose tissue explants was used to study in vitro regulation of adipocyte UCP2 gene expression. A 48-h treatment with BRL49653 and bromopalmitate, two potent activators of PPAR gamma, resulted in a dose-dependent increase in UCP2 mRNA levels. The induction by BRL49653 was rapid (from 6 h) and maintained up to 5 days. TNF alpha provoked a 2-fold decrease in UCP2 mRNA levels. Human recombinant leptin did not affect UCP2 mRNA expression. The data support the hypothesis that fatty acids are involved in the control of adipocyte UCP2 mRNA expression in humans. (C) 1999 Academic Press.

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页码：138 / 141

页数：4

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