Integrated capillary fluorescence DNA biosensor

被引:18
作者
Breimer, MA [1 ]
Gelfand, Y [1 ]
Sadik, OA [1 ]
机构
[1] SUNY Binghamton, Dept Chem, Binghamton, NY 13902 USA
基金
美国国家科学基金会;
关键词
capillary fluorescence; DNA; biosensor;
D O I
10.1016/S0956-5663(02)00248-8
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Covalent attachment of dsDNA molecules inside a glass capillary without the need for hybridization is described. It is shown that the glass capillary has a surface density of 2.5 X 10(13) molecules/cm(2) with specific binding capacity of 62.5%. The resulting substrate was used to develop a biosensor for determining fluorescent organic analytes and metal binding with DNA. The biosensor combines highly specific immobilization chemistry with a capillary-geometry flow cell arrangement. The results show that fluorescent dyes are retained in the dsDNA-modified surface and that exposure to concentrations of nickel and lead ions resulted in a recoverable, highly reproducible diminishment of the fluorescence intensity. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:1135 / 1147
页数:13
相关论文
共 40 条
  • [1] Hybridization assay at a disposable electrochemical biosensor for the attomole detection of amplified human cytomegalovirus DNA
    Azek, F
    Grossiord, C
    Joannes, M
    Limoges, B
    Brossier, P
    [J]. ANALYTICAL BIOCHEMISTRY, 2000, 284 (01) : 107 - 113
  • [2] Babkina SS, 1998, APPL BIOCHEM MICRO+, V34, P520
  • [3] FLUORESCENCE ENERGY-TRANSFER CYANINE HETERODIMERS WITH HIGH-AFFINITY FOR DOUBLE-STRANDED DNA .1. SYNTHESIS AND SPECTROSCOPIC PROPERTIES
    BENSON, SC
    ZENG, ZX
    GLAZER, AN
    [J]. ANALYTICAL BIOCHEMISTRY, 1995, 231 (01) : 247 - 255
  • [4] USE OF THIOL-TERMINAL SILANES AND HETEROBIFUNCTIONAL CROSSLINKERS FOR IMMOBILIZATION OF ANTIBODIES ON SILICA SURFACES
    BHATIA, SK
    SHRIVERLAKE, LC
    PRIOR, KJ
    GEORGER, JH
    CALVERT, JM
    BREDEHORST, R
    LIGLER, FS
    [J]. ANALYTICAL BIOCHEMISTRY, 1989, 178 (02) : 408 - 413
  • [5] *CDC, 1991, PREV LEAD POIS YOUNG
  • [6] Electrochemical DNA biosensor for environmental monitoring
    Chiti, G
    Marrazza, G
    Mascini, M
    [J]. ANALYTICA CHIMICA ACTA, 2001, 427 (02) : 155 - 164
  • [7] Comparison of chemical cleaning methods of glass in preparation for silanization
    Cras, JJ
    Rowe-Taitt, CA
    Nivens, DA
    Ligler, FS
    [J]. BIOSENSORS & BIOELECTRONICS, 1999, 14 (8-9) : 683 - 688
  • [8] Erdem A, 1999, ELECTROANAL, V11, P1372, DOI 10.1002/(SICI)1521-4109(199912)11:18<1372::AID-ELAN1372>3.0.CO
  • [9] 2-4
  • [10] Interaction of the anticancer drug epirubicin with DNA
    Erdem, A
    Ozsoz, M
    [J]. ANALYTICA CHIMICA ACTA, 2001, 437 (01) : 107 - 114