Improved molecular detection of dengue virus serotype 1 variants

被引:38
作者
Reynes, JM
Ong, S
Mey, C
Ngan, C
Hoyer, S
Sall, AA
机构
[1] Inst Pasteur Cambodge, Phnom Penh, Cambodia
[2] Minist Hlth, Natl Dengue Control Programme, Phnom Penh, Cambodia
[3] World Hlth Org, Phnom Penh, Cambodia
关键词
D O I
10.1128/JCM.41.8.3864-3867.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The dengue virus molecular typing method described by Lanciotti and coworkers (R. S. Lanciotti, C. H. Calisher, D. J. Gubler, G. J. Chang, and A. Vance-Vorndam, J. Clin. Microbiol. 30:545-551, 1992) is used worldwide for diagnosis and surveillance. However, it failed to detect DENV-1 variants in Cambodia due to a point mutation. We describe an improvement of the method that allows the detection of additional DENV-1 strains, including potential variants.
引用
收藏
页码:3864 / 3867
页数:4
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