Homogeneity, transport, and signal properties of single Ag particles studied by single-molecule surface-enhanced resonance Raman scattering

被引:62
作者
Eggeling, C
Schaffer, J
Seidel, CAM
Korte, J
Brehm, G
Schneider, S
Schrof, W
机构
[1] Max Planck Inst Biophys Chem, D-37077 Gottingen, Germany
[2] Univ Erlangen Nurnberg, Inst Phys & Theoret Chem, D-91058 Erlangen, Germany
[3] BASF AG, ZKM, Dept Polymer & Solid State Phys, D-67056 Ludwigshafen, Germany
关键词
D O I
10.1021/jp002552+
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
We extended the sensitivity of Raman correlation spectroscopy in solution to the single-molecule level by applying surface- and resonance-enhanced Raman scattering (SERRS) combined with time-gated, confocal signal detection. The brightness of the SERRS signal of single Rhodamine 6G molecules adsorbed on a single silver nanoparticle is comparable to fluorescence. Rare event analysis reveals the existence of few particles with simultaneous SERRS and fluorescence signal. The observation of a dynamic exchange between heterogeneous binding sites is supported by the existence of multiple SERRS brightnesses in the signal intensity distribution and by signal fluctuations in the 60 mus time range detected by autocorrelation analysis. Finally, polarization-dependent SERRS autocorrelation curves and single-particle analysis allowed us to measure individual rotational diffusion times and to directly analyze the heterogeneity of the ensemble in solution.
引用
收藏
页码:3673 / 3679
页数:7
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