Fluorescence resonance energy transfer terminators for DNA sequencing

被引：28

作者：

Nampalli, S ^{[1
]}

Khot, M ^{[1
]}

Kumar, S ^{[1
]}

机构：

[1] Amersham Pharmacia Biotech, Nucle Acid Chem, Piscataway, NJ 08855 USA

来源：

TETRAHEDRON LETTERS | 2000年 / 41卷 / 46期

关键词：

D O I：

10.1016/S0040-4039(00)01617-8

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A four-colour set of fluorescence resonance energy transfer dideoxy nucleotide terminators (10-13), have been synthesised using a rigid and linear tri-functional molecule (2), synthesised via Heck coupling reaction of t-Boc-L-4-iodophenylalanine (1) with N-TFA-propargylamine. Evaluation of the terminators in DNA sequencing reactions, in combination with Thermo Sequenase(TM) II DNA polymerase, demonstrated them to be excellent reagents for high-throughput DNA sequencing. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：8867 / 8871

页数：5

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