Dual laccase-tyrosinase based sonogel-carbon biosensor for monitoring polyphenols in beers

被引:57
作者
Elkaoutit, Mohammed
Naranjo-Rodriguez, Ignacio
Temsamani, Khalid Riffi
De La Vega, Manuel Dominguez
Hidalgo-Hidalgo De Cisneros, Jose Luis
机构
[1] Univ Cadiz, Dept Quim Analit, Fac Ciencias, Cadiz 11510, Spain
[2] Univ Cadiz, Dept Fis Mat Condensada, Fac Ciencias, Cadiz, Spain
关键词
sonogel-carbon electrode; amperometric biosensor; signal bioamplification; beers; polyphenols; bioelectrochemical index;
D O I
10.1021/jf0711136
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
A biosensor based on the bi-immobilization of laccase and tyrosinase phenoloxidase enzymes has been successfully developed. This biosensor employs as the electrochemical transducer the Sonogel-Carbon, a novel type of electrode developed by our group. The immobilization step was accomplished by doping the electrode surface with a mixture of the enzymes, glutaricclialdehyde, and Nafion-ion exchanger, as protective additive. The response of this biosensor, denoted the dual Trametes versicolor laccase (La) and Mushroom tyrosinase (Ty) based Sonogel-Carbon, was optimized directly in beer real samples and its analytical performance with respect to five individual polyphenols was evaluated. The Lac-Ty/Sonogel-Carbon electrode responds to nanomolar concentrations of flavan-3-ols, hydroxycinnamic acids, and hydroxybenzoic acids. The limit of detection, sensitivity, and linear range for caffeic acid, taken as an example, were 26 nM, 167.53 nA M-1, and 0.01-2 mu M, respectively. In addition, the stability and reproducibility of the biosensor were also evaluated in beer samples. The Lac-Ty/sonogel-carbon electrode was verified as very stable in this matrix, maintaining 80% of its stable response for at least three weeks, with a RSD of 3.6% (n = 10). The biosensor was applied to estimate the total polyphenol index in ten beer samples, and a correlation of 0.99 was obtained when the results were compared with those obtained using the Folin-Ciocalteau reagent.
引用
收藏
页码:8011 / 8018
页数:8
相关论文
共 55 条
[2]   Preparation of biosensors by immobilization of polyphenol oxidase in conducting copolymers and their use in determination of phenolic compounds in red wine [J].
Boyukbayram, A. Efif ;
Kiralp, Senem ;
Toppare, Levent ;
Yagci, Yusuf .
BIOELECTROCHEMISTRY, 2006, 69 (02) :164-171
[3]   BIOCATALYSIS WITH POLYPHENOL OXIDASE - A REVIEW [J].
BURTON, SG .
CATALYSIS TODAY, 1994, 22 (03) :459-487
[4]   Monitoring the rancidification process in olive oils using a biosensor operating in organic solvents [J].
Campanella, L ;
Favero, G ;
Pastorino, M ;
Tomassetti, M .
BIOSENSORS & BIOELECTRONICS, 1999, 14 (02) :179-186
[5]   Disposable tyrosinase-peroxidase bi-enzyme sensor for amperometric detection of phenols [J].
Chang, SC ;
Rawson, K ;
McNeil, CJ .
BIOSENSORS & BIOELECTRONICS, 2002, 17 (11-12) :1015-1023
[6]   Study of the responses of a sonogel-carbon electrode towards phenolic compounds [J].
Cordero-Rando, MD ;
Naranjo-Rodríguez, I ;
Palacios-Santander, JM ;
Cubillana-Aguilera, LM ;
Hidalgo-Hidalgo-de-Cisneros, JL .
ELECTROANALYSIS, 2005, 17 (09) :806-814
[7]   The Sonogel-Carbon electrode as a sol-gel graphite-based electrode [J].
Cordero-Rando, MDM ;
de Cisneros, JLHH ;
Blanco, E ;
Naranjo-Rodríguez, I .
ANALYTICAL CHEMISTRY, 2002, 74 (10) :2423-2427
[8]   Analysis of beer components by capillary electrophoretic methods [J].
Cortacero-Ramírez, S ;
de Castro, MHB ;
Segura-Carretero, A ;
Cruces-Blanco, C ;
Fernández-Gutiérrez, A .
TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2003, 22 (07) :440-455
[9]   An bienzyme electrode (alkaline phosphatase polyphenol oxidase) for the amperometric determination of phosphate [J].
Cosnier, S ;
Gondran, C ;
Watelet, JC ;
De Giovani, W ;
Furriel, RPM ;
Leone, FA .
ANALYTICAL CHEMISTRY, 1998, 70 (18) :3952-3956
[10]   A NEW STRATEGY FOR THE CONSTRUCTION OF A TYROSINASE-BASED AMPEROMETRIC PHENOL AND O-DIPHENOL SENSOR [J].
COSNIER, S ;
INNOCENT, C .
BIOELECTROCHEMISTRY AND BIOENERGETICS, 1993, 31 (02) :147-160