Biliary lipid secretion, bile acid metabolism, and gallstone formation are not impaired in hepatic lipase-deficient mice

被引:14
作者
Amigo, L [1 ]
Mardones, P [1 ]
Ferrada, C [1 ]
Zanlungo, S [1 ]
Nervi, F [1 ]
Miquel, JF [1 ]
Rigotti, A [1 ]
机构
[1] Pontificia Univ Catolica Chile, Fac Med, Dept Gastroenterol, Santiago, Chile
关键词
D O I
10.1053/jhep.2003.50379
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Whereas hepatic lipase (HL) has been implicated in lipoprotein metabolism and atherosclerosis, its role in controlling biliary lipid physiology has not been reported. This work characterizes plasma lipoprotein cholesterol, hepatic cholesterol content, bile acid metabolism, biliary cholesterol secretion, and gallstone formation in HL-deficient mice and C57BL/6 controls fed standard chow, a cholesterol-supplemented diet, or a lithogenic diet. Compared with C57BL/6 controls, HL knockout mice exhibited increased basal plasma high-density lipoprotein (HDL) cholesterol as well as reduced cholesterol levels transported in large lipoproteins in response to cholesterol-enriched diets. Hepatic cholesterol content and biliary cholesterol secretion of chow-fed HL knockout and wild-type mice were not different and increased similarly in both strains after feeding dietary cholesterol or a lithogenic diet. There were no differences in biliary bile acid secretion, bile acid pool size and composition, or fecal bile acid excretion between HL-deficient and control mice. HL knockout mice had a similar prevalence of gallstone formation as compared with control mice when both strains were fed with a lithogenic diet. In conclusion, the deficiency of HL has no major impact on the availability of lipoprotein-derived hepatic cholesterol for biliary secretion; HL expression is not essential for diet-induced gallstone formation in mice.
引用
收藏
页码:726 / 734
页数:9
相关论文
共 37 条
[1]   Hepatic overexpression of sterol carrier protein-2 inhibits VLDL production and reciprocally enhances biliary lipid secretion [J].
Amigo, L ;
Zanlungo, S ;
Miquel, JF ;
Glick, JM ;
Hyogo, H ;
Cohen, DE ;
Rigotti, A ;
Nervi, F .
JOURNAL OF LIPID RESEARCH, 2003, 44 (02) :399-407
[2]   Impaired biliary cholesterol secretion and decreased gallstone formation in apolipoprotein E-deficient mice fed a high-cholesterol diet [J].
Amigo, L ;
Quiñones, V ;
Mardones, P ;
Zanlungo, S ;
Miquel, JF ;
Nervi, F ;
Rigotti, A .
GASTROENTEROLOGY, 2000, 118 (04) :772-779
[3]   Hepatic lipase gene therapy in hepatic lipase-deficient mice - Adenovirus-mediated replacement of a lipolytic enzyme to the vascular endothelium [J].
ApplebaumBowden, D ;
Kobayashi, J ;
Kashyap, VS ;
Brown, DR ;
Berard, A ;
Meyn, S ;
Parrott, C ;
Maeda, N ;
Shamburek, R ;
Brewer, HB ;
SantamarinaFojo, S .
JOURNAL OF CLINICAL INVESTIGATION, 1996, 97 (03) :799-805
[4]  
AUWERX JH, 1990, AM J HUM GENET, V46, P470
[5]  
BAMBERGER M, 1983, J LIPID RES, V24, P869
[6]  
BASCHI S, 1998, BIOCHIM BIOPHYS ACTA, V1392, P276
[7]   THE ROLE OF LIPOPROTEIN CHOLESTEROL IN BILIARY STEROID-SECRETION, STUDIES WITH IN-VIVO EXPERIMENTAL-MODELS [J].
BOTHAM, KM ;
BRAVO, E .
PROGRESS IN LIPID RESEARCH, 1995, 34 (01) :71-97
[8]   LIPOPROTEIN ABNORMALITIES ASSOCIATED WITH A FAMILIAL DEFICIENCY OF HEPATIC LIPASE [J].
BRECKENRIDGE, WC ;
LITTLE, JA ;
ALAUPOVIC, P ;
WANG, CS ;
KUKSIS, A ;
KAKIS, G ;
LINDGREN, F ;
GARDINER, G .
ATHEROSCLEROSIS, 1982, 45 (02) :161-179
[9]  
BUSCH SJ, 1994, J BIOL CHEM, V269, P16376
[10]  
Carey Martin C., 1994, P719