Stabilization of peptide guinea pig myelin basic protein 72-85 by N-terminal acetylation-implications for immunological studies

被引:10
作者
de Haan, EC
Wauben, MHM
Wagenaar-Hilbers, JPA
Grosfeld-Stulemeyer, MC
Rijkers, DTS
Moret, EE
Liskamp, RMJ
机构
[1] Univ Utrecht, Utrecht Inst Pharmaceut Sci, Dept Med Chem, NL-3508 TB Utrecht, Netherlands
[2] Univ Utrecht, Dept Infect Dis & Immunol, Div Immunol, NL-3508 TB Utrecht, Netherlands
关键词
acetylation; experimental autoimmune encephalomyelitis EAE; major histocompatibility complex; MHC; myelin basic protein; MBR; peptide; pyroglutamic acid;
D O I
10.1016/j.molimm.2003.10.015
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Peptide gpMBP72-85, containing amino acids 72-85 of guinea pig myelin basic protein is commonly used to induce experimental autoimmune encephalomyelitis in Lewis rats. The N-terminal glutamine in this peptide can cyclize to pyroglutamic acid, leading to loss of the first MHC anchor for binding to MHC class II. Acetylation of the peptide N-terminus prevents pyroglutamic acid formation and ensures a constant quality. An increased MHC binding affinity after N-terminal acetylation was observed. This modification also enhanced T cell proliferation of a gpMBP reactive T cell clone. The encephalitogenicity of peptide gpMBP72-85 was unaffected by acetylation. It is concluded that acetylation improves the chemical stability of -pMBP72-85, and is not detrimental but rather favorable for its biochemical and immunological. in vitro. and in vivo behavior. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码:943 / 948
页数:6
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