Antagonistic effects of leucine and glutamine on the mTOR pathway in myogenic C2C12 cells

被引：59

作者：

Deldicque, L. ^{[1
]}

Canedo, C. Sanchez ^{[2
]}

Horman, S. ^{[3
]}

De Potter, I. ^{[3
]}

Bertrand, L. ^{[2
]}

Hue, L. ^{[3
]}

Francaux, M. ^{[1
]}

机构：

[1] Univ Catholique Louvain, Dept Educ Phys & Readaptat, B-1348 Louvain, Belgium

[2] Univ Catholique Louvain, Div Cardiol, B-1200 Brussels, Belgium

[3] Univ Catholique Louvain, Inst Pathol Cellulaire Christian Duve, Unite Hormones & Metab, B-1200 Brussels, Belgium

来源：

AMINO ACIDS | 2008年 / 35卷 / 01期

关键词：

amino acids; p70(S6k); 4E-BP1; protein synthesis; gene expression;

D O I：

10.1007/s00726-007-0607-z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study compared the effects of leucine and glutamine on the mTOR pathway, on protein synthesis and on muscle-specific gene expression in myogenic C2C12 cells. Leucine increased the phosphorylation state of mTOR, on both Ser2448 and Ser2481, and its downstream effectors, p70(S6k), S6 and 4E-BP1. By contrast, glutamine decreased the phosphorylation state of mTOR on Ser2448, p70(S6k) and 4E-BP1, but did not modify the phosphorylation state of mTOR on Ser2481 and S6. Whilst the phosphorylation state of the mTOR pathway is usually related to protein synthesis, the incorporation of labelled methionine/cysteine was only transiently modified by leucine and was unaltered by glutamine. However, these two amino acids affected the mRNA levels of desmin, myogenin and myosin heavy chain in a time-dependant manner. In conclusion, leucine and glutamine have opposite effects on the mTOR pathway. Moreover, they induce modification of muscle-specific gene expression, unrelated to their effects on the mTOR/p70(S6k) pathway.

引用

页码：147 / 155

页数：9

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