Cutting edge: CCR4 mediates antigen-primed T cell binding to activated dendritic cells

被引：40

作者：

Wu, MT ^{[1
]}

Fang, H ^{[1
]}

Hwang, ST ^{[1
]}

机构：

[1] NCI, Dermatol Branch, Bethesda, MD 20892 USA

来源：

JOURNAL OF IMMUNOLOGY | 2001年 / 167卷 / 09期

关键词：

D O I：

10.4049/jimmunol.167.9.4791

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The binding of a T cell to an Ag-laden dendritic cell (DC) is a critical step of the acquired immune response. Herein, we address whether a DC-produced chemokine can induce the arrest of T cells on DC under dynamic flow conditions. Ag-primed T cells and a T cell line were observed to rapidly (similar to0.5 s) bind to immobilized DC at low shear stress (0.1-0.2 dynes/cm(2)) in a pertussis toxin-sensitive fashion. Quantitatively, Ag-primed T cells displayed 2- to 3-fold enhanced binding to DC compared with unprimed T cells (p < 0.01). In contrast to naive T cells, primed T cell arrest was largely inhibited by pertussis toxin, neutralization of the CC chemokine, macrophage-derived chemokine (CCL22), or by desensitization of the CCL22 receptor, CCR4. Our results demonstrate that DC-derived CCL22 induces rapid binding of activated T cells under dynamic conditions and that Ag-primed and naive T cells fundamentally differ with respect. to chemokine-dependent binding to DC.

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收藏

页码：4791 / 4795

页数：5

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