Ultrastructure of gametes after intracytoplasmic sperm injection

被引:30
作者
Bourgain, C
Nagy, ZP
De Zutter, H
Van Ranst, H
Nogueira, D
Van Steirteghem, AC
机构
[1] Dutch Speaking Brussels Free Univ, Dept Pathol, B-1090 Brussels, Belgium
[2] Dutch Speaking Brussels Free Univ, Univ Hosp, Ctr Reprod Med, B-1090 Brussels, Belgium
关键词
intracytoplasmic sperm injection; oocytes; spermatozoa; ultrastructure;
D O I
10.1093/humrep/13.suppl_1.107
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
The ultrastructure of 18 metaphase-II, oocytes after intracytoplasmic sperm injection (ICSI) was analysed from 30 min to 8 h following the microinjection, Three control metaphase-II oocytes were not injected. The 21 oocytes were embedded individually and examined in serial pole-to-pole sections. Seventeen oocytes had been matured in vitro from the germinal vesicle stage and showed ultrastructural alterations due to long-term culture, In microinjected oocytes, membrane-bound vacuoles and oolemma inclusions were found at the injection site, The ICSI oocytes showed evidence of plasma-membrane damage and increased oocyte exchange processes with residual and multivesiculated bodies. Gamete membrane fusion was not observed. Acrosome reaction was observed within the ooplasm 15 min after ICSI, Sperm elements were not incorporated into any oocyte vacuole, Onset of oocyte activation was associated with sperm chromatin decondensation, Cortical granules were released at 60 min and two pronuclei mere formed at 6 h after ICSI, The male and female pronucleus formation was asynchronous, The findings suggest specific ICSI-related morphological features of early fertilization.
引用
收藏
页码:107 / 116
页数:10
相关论文
共 24 条
[1]   EVALUATION OF THE MEIOTIC SPINDLE APPARATUS IN OOCYTES UNDERGOING INTRACYTOPLASMIC SPERM INJECTION [J].
ASADA, Y ;
BAKA, SG ;
HODGEN, GD ;
LANZENDORF, SE .
FERTILITY AND STERILITY, 1995, 64 (02) :376-381
[2]   BEHAVIOR OF SPERMATOZOA IN HUMAN OOCYTES DISPLAYING NO OR ONE PRONUCLEUS AFTER INTRACYTOPLASMIC SPERM INJECTION [J].
DOZORTSEV, D ;
DESUTTER, P ;
DHONT, M .
HUMAN REPRODUCTION, 1994, 9 (11) :2139-2144
[3]  
EICHENLAUB RITT.U, 1995, HUM GENET, V80, P259
[4]  
EICHENLAUBRITTE.U, 1988, HUM REPROD, V10, P2343
[5]   INTRACYTOPLASMIC SPERM INJECTION IN MICE - INCREASED FERTILIZATION AND DEVELOPMENT TO TERM AFTER INDUCTION OF THE ACROSOME REACTION [J].
LACHAMKAPLAN, O ;
TROUNSON, A .
HUMAN REPRODUCTION, 1995, 10 (10) :2642-2649
[6]  
LASSALLE B, 1991, J REPROD FERTIL, V91, P393
[7]  
LOPATA A, 1980, FERTIL STERIL, V33, P12
[8]   TIME-COURSE OF OOCYTE ACTIVATION, PRONUCLEUS FORMATION AND CLEAVAGE IN HUMAN OOCYTES FERTILIZED BY INTRACYTOPLASMIC SPERM INJECTION [J].
NAGY, ZP ;
LIU, J ;
JORIS, H ;
DEVROEY, P ;
VANSTEIRTEGHEM, A .
HUMAN REPRODUCTION, 1994, 9 (09) :1743-1748
[9]   Pregnancy and birth after intracytoplasmic sperm injection of in vitro matured germinal-vesicle stage oocytes: Case report [J].
Nagy, ZP ;
Cecile, J ;
Liu, J ;
Loccufier, A ;
Devroey, P ;
VanSteirteghem, A .
FERTILITY AND STERILITY, 1996, 65 (05) :1047-1050
[10]   EARLY SPERM-EGG INTERACTION AFTER SPERM MICROINJECTION [J].
SATHANANTHAN, AH ;
NG, SC ;
LIOW, SL ;
HO, J ;
BONGSO, A ;
RATNAM, SS .
REPRODUCTION FERTILITY AND DEVELOPMENT, 1994, 6 (01) :105-111