The gene for component-II of botulinum C2 toxin

被引：34

作者：

Kimura, K

Kubota, T

Ohishi, I

Isogai, E

Isogai, H

Fujii, N

机构：

[1] Sapporo Med Univ, Sch Med, Dept Microbiol, Sapporo, Hokkaido 060, Japan

[2] Nippon Vet & Anim Sci Univ, Div Vet Biochem, Musashino, Tokyo 180, Japan

[3] Hlth Sci Univ Hokkaido, Dept Prevent Dent, Ishikari, Hokkaido 1757, Japan

[4] Sapporo Med Univ, Sch Med, Ctr Anim Experimentat, Sapporo, Hokkaido 060, Japan

来源：

VETERINARY MICROBIOLOGY | 1998年 / 62卷 / 01期

关键词：

Clostridium botulinum; toxins; gene cloning; component-II;

D O I：

10.1016/S0378-1135(98)00195-3

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The gene encoding component-II of the Clostridium botulinum C2 toxin was cloned from the chromosomal DNA of C, botulinum type C strain (C)-203U28, and the nucleotide sequence was determined. The gene (bc2h) encodes a protein with 721 amino acid residues and is located at 247 bp downstream of the gene for component-I. The N-terminal 16 amino acids were identical to those obtained by analysis of the purified component-II toxin. The ORF for bc2h had only 39% homology at the amino acid level with the C. perfringens iota-Ib protein and an ATP/GTP binding site which is present in the iota-Ib protein is missing from the protein encoded by bc2h. Both genes had a homologous region that predicts a transmembrane segment. (C) 1998 Elsevier Science B,V. All rights reserved.

引用

页码：27 / 34

页数：8

共 13 条

[1] BOTULINUM-C2 TOXIN ADP-RIBOSYLATES ACTIN [J].