Newcastle disease virus: detection and characterization by PCR of recent German isolates differing in pathogenicity

被引：59

作者：

Oberdorfer, A ^{[1
]}

Werner, O ^{[1
]}

机构：

[1] Fed Res Ctr Virus Dis Anim, Friedrich Loeffler Inst, Inst Appl Virol, D-17498 Insel Riems, Germany

来源：

AVIAN PATHOLOGY | 1998年 / 27卷 / 03期

关键词：

D O I：

10.1080/03079459808419330

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The fusion (F) protein plays an important role in determining the virulence of Newcastle disease virus (NDV) strains. A reverse transcriptase-polymerase chain reaction (RT-PCR) is described which amplifies a 362 bp fragment encompassing the region of the F protein most important for pathogenicity. A specific PCR product was obtained independent of strain, pathogenicity and host of origin. Sequencing of the region specifying the F protein cleavage site confirmed the correlation between deduced amino acid sequence and pathogenicity. Oligonucleotides corresponding to the sequence of the pathospecific region were designed for recent German NDV isolates and labelled with digoxigenin. Hybridization of PCR fragments of different isolates with pathotype-specific oligonucleotides allowed an estimation of the pathogenicity of most isolates. Results were in good agreement with experimentally determined ICPI values.

引用

页码：237 / 243

页数：7

共 23 条

[1]

Alexander D.J., 1989, LAB MANUAL ISOLATION, V3rd, P114

[2] Identification and grouping of Newcastle disease virus strains by restriction site analysis of a region from the F gene [J].