Formation of colloidal silver nanoparticles stabilized by Na+-poly(γ-glutamic acid)-silver nitrate complex via chemical reduction process

被引：179

作者：

Yu, Da-Guang ^{[1
]}

机构：

[1] Nanya Inst Technol, Dept Text Sci, Chungli, Taiwan

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2007年 / 59卷 / 02期

关键词：

silver nanoparticle; poly(gamma-glutamic acid); antibacterial activity; cytotoxicity;

D O I：

10.1016/j.colsurfb.2007.05.007

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Macromolecular and polyanionic Na+-poly(gamma-glutamic acid) (PGA) silver nitrate complex acted as both a metal ion provider and a particle protector to fabricate nanosized silver colloids under chemical reduction by dextrose. The formation and size of particles have been characterized from transmission electron microscopy (TEM), dynamic light scattering analysis and UV-vis spectrophotometer. The results showed that the average particle size was 17.2 +/- 13.4 to 37.3 +/- 5.5 nm, apparently depending on the complex concentration. It was found that the rate constant and conversion of silver nanoparticles were proportional to the concentration of PGA. The growth mechanism of nanosized silver colloid was fully discussed. In addition, the in vitro cytotoxicity evaluated by L929 fibroblasts proliferation and antibacterial activity against Gram-positive strain (methicillin-resistant S. aureus (MRSA)) and Gram-negative strain (P. aeruginosa) bacteria have been assessed. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：171 / 178

页数：8

共 29 条

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