Structural diversification and neo-functionalization during floral MADS-box gene evolution by C-terminal frameshift mutations

被引:209
作者
Vandenbussche, M
Theissen, G
Van de Peer, Y
Gerats, T
机构
[1] State Univ Ghent VIB, Dept Plant Syst Biol, B-9000 Ghent, Belgium
[2] Univ Jena, Lehrstuhl Genet, D-07743 Jena, Germany
关键词
D O I
10.1093/nar/gkg642
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Frameshift mutations generally result in loss-of-function changes since they drastically alter the protein sequence downstream of the frameshift site, besides creating premature stop codons. Here we present data suggesting that frameshift mutations in the C-terminal domain of specific ancestral MADS-box genes may have contributed to the structural and functional divergence of the MADS-box gene family. We have identified putative frameshift mutations in the conserved C-terminal motifs of the B-function DEF/AP3 subfamily, the A-function SQUA/AP1 subfamily and the E-function AGL2 subfamily, which are all involved in the specification of organ identity during flower development. The newly evolved C-terminal motifs are highly conserved, suggesting a de novo generation of functionality. Interestingly, since the new C-terminal motifs in the A- and B-function subfamilies are only found in higher eudicotyledonous flowering plants, the emergence of these two C-terminal changes coincides with the origin of a highly standardized floral structure. We speculate that the frameshift mutations described here are examples of co-evolution of the different components of a single transcription factor complex. 3' terminal frameshift mutations might provide an important but so far unrecognized mechanism to generate novel functional C-terminal motifs instrumental to the functional diversification of transcription factor families.
引用
收藏
页码:4401 / 4409
页数:9
相关论文
共 43 条
[1]   COMPLEMENTARY FLORAL HOMEOTIC PHENOTYPES RESULT FROM OPPOSITE ORIENTATIONS OF A TRANSPOSON AT THE PLENA-LOCUS OF ANTIRRHINUM [J].
BRADLEY, D ;
CARPENTER, R ;
SOMMER, H ;
HARTLEY, N ;
COEN, E .
CELL, 1993, 72 (01) :85-95
[2]   FLORAL HOMEOTIC MUTATIONS PRODUCED BY TRANSPOSON-MUTAGENESIS IN ANTIRRHINUM-MAJUS [J].
CARPENTER, R ;
COEN, ES .
GENES & DEVELOPMENT, 1990, 4 (09) :1483-1493
[3]   Analysis of the C-terminal region of Arabidopsis thaliana APETALA1 as a transcription activation domain [J].
Cho, SC ;
Jang, SH ;
Chae, SJ ;
Chung, KM ;
Moon, YH ;
An, GH ;
Jang, SK .
PLANT MOLECULAR BIOLOGY, 1999, 40 (03) :419-429
[4]  
Coen Enrico S., 1992, Current Opinion in Cell Biology, V4, P929, DOI 10.1016/0955-0674(92)90120-2
[5]   THE WAR OF THE WHORLS - GENETIC INTERACTIONS CONTROLLING FLOWER DEVELOPMENT [J].
COEN, ES ;
MEYEROWITZ, EM .
NATURE, 1991, 353 (6339) :31-37
[6]  
Davies B, 1994, Results Probl Cell Differ, V20, P235
[7]   Transcriptional regulators and the evolution of plant form [J].
Doebley, J ;
Lukens, L .
PLANT CELL, 1998, 10 (07) :1075-1082
[8]   Ternary complex formation between the MADS-box proteins SQUAMOSA, DEFICIENS and GLOBOSA is involved in the control of floral architecture in Antirrhinum majus [J].
Egea-Cortines, M ;
Saedler, H ;
Sommer, H .
EMBO JOURNAL, 1999, 18 (19) :5370-5379
[9]  
Force A, 1999, GENETICS, V151, P1531
[10]   Evolution of a transcriptional repression domain in an insect Hox protein [J].
Galant, R ;
Carroll, SB .
NATURE, 2002, 415 (6874) :910-913