Local viscosity probed by photonic force microscopy

被引：98

作者：

Pralle, A ^{[1
]}

Florin, EL ^{[1
]}

Stelzer, EHK ^{[1
]}

Horber, JKH ^{[1
]}

机构：

[1] European Mol Biol Lab, Cell Biophys Programme, D-69117 Heidelberg, Germany

来源：

APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING | 1998年 / 66卷 / Suppl 1期

关键词：

Viscosity; Microscopy; Diffusion Coefficient; Viscous Drag; Surface Separation;

D O I：

10.1007/s003390051102

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

The Brownian motion of 0.5 mu m and 1 mu m polystyrene spheres used as probes for a photonic force microscope is recorded at various distances from a surface with nanometer spatial and 25 mu s temporal resolution. Analyzing time-windows of 0.4s of the Brownian motion, the diffusion constant and local viscosity are determined. Using 0.5 mu m spheres the viscosity can be measured in volumes as small as 1 mu m(3) with +/-15% precision within 0.4 s. The decrease of the diffusion coefficient due to the partial confinement near a surface is measured continuously over sphere-surface separations from 3 mu m down to 200 nm. The increase in viscous drag is found to agree with Brenner's analytical result [1].

引用

页码：S71 / S73

页数：3

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