Host protection against deliberate bacterial contamination of an extracellular matrix bioscaffold versus Dacron™ mesh in a dog model of orthopedic soft tissue repair

被引:42
作者
Badylak, SF
Wu, CC
Bible, M
McPherson, E
机构
[1] McGowan Inst Regenerat Med, Pittsburgh, PA 15219 USA
[2] Univ Pittsburgh, Dept Surg, Pittsburgh, PA 15219 USA
[3] Purdue Univ, Anim Dis Diagnost Lab, W Lafayette, IN 47907 USA
[4] Univ So Calif, Keck Sch Med, Dept Orthopaed Surg, Los Angeles, CA 90089 USA
关键词
ECM-extracellular matrix; infection resistance; scaffold; orthopedic surgery; biomaterial;
D O I
10.1002/jbm.b.10062
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The resistance of two biomaterials, one synthetic and one biologic in origin, to deliberate bacterial infection was compared in a dog model of orthopedic soft tissue reconstruction. Twenty-four adult female dogs were randomly divided into two equal groups and a 2.0-cm-round full-thickness defect was created on the lateral surface of the stifle joint, leaving only the synovium and skin intact. The defect was surgically repaired with either Dacron(TM) mesh or a porcine derived extracellular matrix (ECM) scaffold material. The repair site was inoculated with 1 X 108 Staphylococcus aureus at the time of surgery and the dogs were survived for 28 days. Results showed a chronic pyogranulomatous inflammatory response at the Dacron(TM) implant sites versus a constructive tissue-remodeling response without residual inflammation at the ECM implant site. Three dogs in the group receiving the Dacron(TM) mesh were treated with Keflex(TM) (500 mg bid X 7 days) for signs of septicemia. A quantitative bacterial count of the implant sites at the time of sacrifice showed 6.52 x 10(5) +/- 1.2 x 10(6) and 6.5 x 10(2) +/- 1.8 x 10(3) bacteria per gram of tissue for the Dacron(TM) and ECM scaffold sites, respectively (P < .03). The ECM implant material was more resistant than the synthetic implant material to persistent infection following deliberate bacterial contamination and the ECM scaffold supported constructive tissue remodeling. (C) 2003 Wiley Periodicals, Inc.
引用
收藏
页码:648 / 654
页数:7
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