High-resolution genotyping of Campylobacter species by use of PCR and high-throughput mass spectrometry

被引：26

作者：

Hannis, James C. ^{[1
]}

Manalili, Sheri M. ^{[1
]}

Hall, Thomas A. ^{[1
]}

Ranken, Raymond ^{[1
]}

White, Neill ^{[1
]}

Sampath, Rangarajan ^{[1
]}

Blyn, Lawrence B. ^{[1
]}

Ecker, David J. ^{[1
]}

Mandrell, Robert E. ^{[2
]}

Fagerquist, Clifton K. ^{[2
]}

Bates, Anna H. ^{[2
]}

Miller, William G. ^{[2
]}

Hofstadler, Steven A. ^{[1
]}

机构：

[1] ISIS Pharmaceut Inc, Ibis Biosci Inc, Carlsbad, CA 92008 USA

[2] USDA ARS, Western Reg Res Ctr, Produce Safety & Microbiol Res Unit, Albany, CA 94710 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2008年 / 46卷 / 04期

关键词：

D O I：

10.1128/JCM.02158-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In this work we report on a high-throughput mass spectrometry-based technique for the rapid highresolution identification of Campylobacter jejuni strain types. This method readily distinguishes C.jejuni from C. coli, has a resolving power comparable to that of multilocus sequence typing (MLST), is applicable to mixtures, and is highly automated. The strain typing approach is based on high-performance mass spectrometry, which "weighs" PCR amplicons with enough mass accuracy to unambiguously determine the base composition of each amplicon (i.e., the numbers of A's, G's, C's, and T's). Amplicons are derived from PCR primers which amplify short (< 140-bp) regions of the housekeeping genes used by conventional MLST strategies. The results obtained with a challenge panel that comprised 25 strain types of C.jejuni and 25 strain types of C. coli are presented. These samples were parsed and resolved with demonstrated sensitivity down to 10 genomes/PCR from pure isolates.

引用

页码：1220 / 1225

页数：6

共 31 条

[1] Campylobacter jejuni -: An emerging foodborne pathogen [J].

Altekruse, SF ;

Stern, NJ ;

Fields, PI ;

Swerdlow, DL .

EMERGING INFECTIOUS DISEASES, 1999, 5 (01) :28-35

[2] EXTRAINTESTINAL CAMPYLOBACTER-JEJUNI AND CAMPYLOBACTER-COLI INFECTIONS - HOST FACTORS AND STRAIN CHARACTERISTICS [J].

BLASER, MJ ;

PEREZ, GP ;

SMITH, PF ;

PATTON, C ;

TENOVER, FC ;

LASTOVICA, AJ ;

WANG, WL .

JOURNAL OF INFECTIOUS DISEASES, 1986, 153 (03) :552-559

[3] Multilocus sequence typing system for Campylobacter jejuni [J].

Dingle, KE ;

Colles, FM ;

Wareing, DRA ;

Ure, R ;

Fox, AJ ;

Bolton, FE ;

Bootsma, HJ ;

Willems, RJL ;

Urwin, R ;

Maiden, MCJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (01) :14-23

[4] Rapid identification and strain-typing of respiratory pathogens for epidemic surveillance [J].

Ecker, DJ ;

Sampath, R ;

Blyn, LB ;

Eshoo, MW ;

Ivy, C ;

Ecker, JA ;

Libby, B ;

Samant, V ;

Sannes-Lowery, KA ;

Melton, RE ;

Russell, K ;

Freed, N ;

Barrozo, C ;

Wu, JG ;

Rudnick, K ;

Desai, A ;

Moradi, E ;

Knize, DJ ;

Robbins, DW ;

Hannis, JC ;

Harrell, PM ;

Massire, C ;

Hall, TA ;

Jiang, Y ;

Ranken, R ;

Drader, JJ ;

White, N ;

McNeil, JA ;

Crooke, ST ;

Hofstadler, SA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (22) :8012-8017

[5]

Ecker DJea., 2006, JALA J ASS LAB AUTOM, P341, DOI DOI 10.1016/J

[6] Identification of Acinetobacter species and genotyping of Acinetobacter baumannii by multilocus PCR and mass spectrometry [J].

Ecker, Joseph A. ;

Massire, Christian ;

Hall, Thomas A. ;

Ranken, Raymond ;

Pennella, Thuy-Trang D. ;

Ivy, Cristina Agasino ;

Blyn, Lawrence B. ;

Hofstadler, Steven A. ;

Endy, Timothy P. ;

Scott, Paul T. ;

Lindler, Luther ;

Hamilton, Tacita ;

Gaddy, Charla ;

Snow, Kerry ;

Pe, Marie ;

Fishbain, Joel ;

Craft, David ;

Deye, Gregory ;

Riddell, Scott ;

Milstrey, Eric ;

Petruccelli, Bruno ;

Brisse, Sylvain ;

Harpin, Vanessa ;

Schink, Amy ;

Ecker, David J. ;

Sampath, Rangarajan ;

Eshoo, Mark W. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (08) :2921-2932

[7] Genomic and proteomic identification of a DNA-binding protein used in the "fingerprinting" of Campylobacter species and strains by MALDI-TOF-MS protein biornarker analysis [J].

Fagerquist, CK ;

Miller, WG ;

Harden, LA ;

Bates, AH ;

Vensel, WH ;

Wang, GL ;

Mandrell, RE .

ANALYTICAL CHEMISTRY, 2005, 77 (15) :4897-4907

[8]

FRIEDMAN CR, 2000, CAMPYLOBACTER, V1, P121

[9] Public health implications of campylobacter outbreaks in England and Wales, 1995-9: epidemiological and microbiological investigations [J].

Frost, JA ;

Gillespie, IA ;

O'Brien, SJ .

EPIDEMIOLOGY AND INFECTION, 2002, 128 (02) :111-118

[10] A case-case comparison of Campylobacter coli and Campylobacter jejuni infection:: A tool for generating hypotheses [J].

Gillespie, IA ;

O'Brien, SJ ;

Frost, JA ;

Adak, GK ;

Horby, P ;

Swan, AV ;

Painter, MJ ;

Neal, KR .

EMERGING INFECTIOUS DISEASES, 2002, 8 (09) :937-942

← 1 2 3 4 →